Green Synthesis of Silver Nanoparticles from Syzygium cumini (L.) Skeels Seed Extract and their Potential Medicinal Applications.

Aim: Nanotechnology is considered as one of the fastest-developing areas in the biomedicine field. Hence, the green synthesis of silver nanoparticles from Syzygium cumini seed extract was carried out in this study.

Methodology: The synthesized nanoparticles were characterized by UV-Vis spectroscopy, FTIR (Fourier transform infrared), FE-SEM (Field Emission scanning electron microscopic), AFM (Atomic Force Microscope), XRD (X-ray diffraction), and EDX (Energy dispersive X-Ray). Their antioxidant and anti-inflammatory activity were evaluated by DPPH (2,2-diphenyl-1- picrylhydrazyl), PM (Phosphomolybdenum) assay, and albumin denaturation assay. Further, the antibacterial activity of the nanoparticles was studied against Gram-positive and Gram-negative bacteria using the agar well diffusion method. In addition, the antidiabetic activity of nanoparticles was studied by α-amylase and α-glucosidase inhibition assays.

Results: The surface plasmon resonance at 430 nm confirmed the formation of silver nanoparticles. They were stable and spherical in shape, with sizes ranging from 30 to 90 nm. The DPPH inhibition % of silver nanoparticles varied from 7.91±0.39% to 68.35±0.76%. The % inhibition of albumin denaturation was comparable to the diclofenac. Further, the results of antibacterial activity revealed that the zone of inhibition for all the test bacteria varied from 14.33±0.58 to 25.33±0.58 mm, where B. cereus was more susceptible. In addition, the % inhibition of α-amylase and α-glucosidase varied from 19.91±0.15% to 61.43±0.31% and 15.26±0.11% to 55.38±0.20%, respectively.

Conclusion: This study is the first attempt of utilizing the silver nanoparticles synthesized from S. cumini seed extract for antidiabetic activity. The study suggests that these nanoparticles could be well utilized in pharmaceutical industries as an efficient antioxidant, anti-inflammatory, antibacterial, and antidiabetic drug.