EFMouse: a Matlab toolbox to model electric fields in the mouse brain.

Compared to the rapidly growing literature on transcranial electrical stimulation (tES) in humans, research into the mechanisms underlying neuromodulation by tES using in-vivo animal models is growing but still relatively rare. Such research, however, is key to overcoming experimental limitations in humans and essential to build a detailed understanding of the in-vivo consequences of tES that can ultimately lead to development of targeted and effective therapeutic applications of noninvasive brain stimulation. The sheer difference in scale and geometry between animal models and the human brain contributes to the complexity of designing and interpreting animal studies. Here we extend previous approaches to model intracranial electric fields to generate predictions that can be tested with in-vivo intracranial recordings. Although the toolbox has general applicability and could be used to predict intracranial fields for any tES study using mice, we illustrate its usage by comparing fields in a high-density multi-electrode montage with a more traditional two electrode montage. Our simulations show that both montages can produce strong focal homogeneous electric fields in targeted areas. However, the high-density montage produces a field that is more perpendicular to the visual cortical surface, which is expected to result in larger changes in neuronal excitability.