Evaluation of the sperm parameters, oxidative stress, and histopathological effects of vitamin B in preventing -induced testicular toxicity: An experimental study.

Background: () causes sterility by affecting the reproductive system. Vitamin B improves sperm quantity and function.

Objective: Vitamin B protection against adverse effects was investigated.

Materials And Methods: 40 C57 male mice (6 wk) were randomly assigned to 4 equal groups (n = 10) including, group 1 (control without any intervention), group 2 (H), 3 (HP), and 4 (HB) received 1 10 colony forming unit (CFU) of , 1 10 CFU of +phosphate buffered saline, 1 10 CFU of 50 g/kg vitamin B intraperitoneally, respectively. In the induction groups, the was orogasterically injected 3 times with 1 cc phosphate buffered saline throughout the day. Then testicular metrics, sperm motility, viability, quantity, and shape, plasma levels of malondialdehyde (MDA), superoxide dismutase, glutathione peroxidase, and total antioxidant capacity were measured. Also, testicular-tissue changes were examined using Johnson scores, tubular differentiation index, and spermatogenesis index. Vitamin B, homocysteine, and testosterone serum levels were examined.

Results: The results showed a significantly lower Johnson score, tubular differentiation index, and spermatogenesis index, and serum level of testosterone and homocysteine as well as a higher MDA level in the H and HP groups than the HB group (p 0.05). In contrast, the highest superoxide dismutase and glutathione peroxidase enzymes activity and total antioxidant capacity as well as the lowest serum level of MDA were found in the HB group compared to other groups (p 0.05).

Conclusion: Vitamin B increased antioxidant enzyme activity, enhanced sperm parameters, and decreased injury to testicular tissue. It can be used as a potent antioxidant in reducing testicular damage induced by .