Aim: To evaluate the cytotoxic and proapoptotic effects of C6 ceramide on the C6 rat glioma cell line.
Material And Methods: The C6 rat glioma cell line was evaluated. Using a confocal microscope and the appropriate software, the cytotoxic effects of C6 ceramide were identified using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H tetrazolium bromide (MTT) colorimetric experiments. Transmission electron microscopy (TEM) was utilized to examine the ultrastructural changes following treatment with IC50 concentrations of C6 ceramide.
Results: Condensation and fragmentation of nuclei and DNA laddering was observed, indicating apoptotic cell death. C6 ceramide induced apoptosis and effectively caused cytotoxicity in the C6 glioblastoma cells. MTT assay demonstrated > 90% cell death after short-term application of C6 ceramide, confirming its apoptosis-triggering effect. Apoptosis was also confirmed via confocal microscopy and TEM.
Conclusion: Glioblastoma cells undergo apoptosis when exposed to C6 ceramide, which makes it a potential chemotherapeutic agent for the treatment of this aggressive brain cancer.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.5137/1019-5149.JTN.44624-23.3 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Skin Hydration by Natural Moisturizing Factors, a Story of H-Bond Networking.
J Phys Chem B
January 2025
INSERM U1248 Pharmacology & Transplantation, Univ. Limoges, CBRS, 2 Rue du Prof. Descottes, F-87000 Limoges, France.
Dry skin is a common condition that is experienced by many. Besides being particularly present during the cold season, various diseases exist all year round, leading to localized xerosis. To prevent it, the skin is provided with natural moisturizing factors (NMFs).
View Article and Find Full Text PDFDo reproductive endocrinology and infertility fellowship websites reflect a commitment to diversity, equity, and inclusion?
F S Rep
December 2024
Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, University Hospitals Cleveland Medical Center, Beachwood, Ohio.
Objective: To characterize the frequency of diversity elements (DEs) in reproductive endocrinology and infertility (REI) fellowship websites and analyze these elements according to program characteristics.
Design: Forty-nine REI fellowship websites were assessed for 20 DEs that represent programmatic commitments to diversity, equity, and inclusion (DEI). Program websites were categorized by the number of discrete DEs featured: low (0-6); moderate (7-13); or high (14-20).
Oral Lipids/Fatty Acids Supplements and Eczema: What Is Known?
Dermatitis
January 2025
Northwestern University Feinberg School of Medicine, Chicago, Illinois, USA.
The pathogenesis of atopic dermatitis (AD) points to skin barrier dysfunction as a critical piece of the puzzle. Deficiencies in fatty acids and ceramides-key elements of the skin barrier-have been linked to AD. Fatty acids can be separated into omega-3 and omega-6, which can be found in a variety of foods such as fish, nuts, seeds, and even plants.
View Article and Find Full Text PDFSparstolonin B Reduces Estrogen-Dependent Proliferation in Cancer Cells: Possible Role of Ceramide and PI3K/AKT/mTOR Inhibition.
Pharmaceuticals (Basel)
November 2024
Department of Medical Biochemistry, Faculty of Medicine, Akdeniz University, Antalya 07070, Turkey.
The aim of this study was to determine the effect of Sparstolonin B (SsnB) on cell proliferation and apoptosis in human breast cancer (MCF-7) and human ovarian epithelial cancer (OVCAR-3) cell lines in the presence and absence of estradiol hemihydrate (ES). Phosphoinositol-3 kinase (PI3K), phosphorylated protein kinase B alpha (p-AKT), phosphorylated mTOR (mechanistic target of rapamycin) signaling proteins, and sphingomyelin/ceramide metabolites were also measured within the scope of the study. The anti-proliferative effects of SsnB therapy were evaluated over a range of times and concentrations.
View Article and Find Full Text PDFInhibiting De Novo Biosynthesis of Ceramide by L-Cycloserine Can Prevent Light-Induced Retinal Degeneration in Albino BALB/c Mice.
Int J Mol Sci
December 2024
Department of Pharmaceutical Sciences, University of Tennessee Health Science Center, Memphis, TN 38163, USA.
Retinal degenerative diseases lead to irreversible vision loss due to photoreceptor cell death, driven by complex genetic and environmental factors. Ceramide, a sphingolipid metabolite, emerges as a critical mediator in the apoptotic cascade associated with retinal degeneration. Our previous work demonstrated L-Cycloserine's ability to protect photoreceptor-derived cells from oxidative stress by inhibiting the de novo ceramide pathway and thus prompting further investigation on its effect in the in vivo retina.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!