AI Article Synopsis
- Lumpy skin disease (LSD) is a viral illness that primarily affects cattle, caused by a specific poxvirus from the capripoxvirus genus.
- The text outlines a detailed protocol for sequencing the LSD virus genome using an amplicon-based method, including steps like DNA extraction and library preparation.
- This protocol is adaptable to various Illumina sequencing platforms and offers a fast and scalable approach for researchers; for more detailed information, refer to the work by Bhatt et al.
Article Abstract
Lumpy skin disease (LSD) is a viral disease predominantly affecting cattle caused by a poxvirus belonging to the capripoxvirus genus. Here, we present a protocol for next-generation sequencing of the LSD virus genome using an amplicon-based approach. We describe steps for DNA extraction, viral DNA enrichment, amplicon pooling and purification, and library preparation and pooling. We then detail procedures for sequencing and computational analysis. This protocol can be adapted to any Illumina sequencing platform as an accelerated and scalable system. For complete details on the use and execution of this protocol, please refer to Bhatt et al..
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11342167 | PMC |
| http://dx.doi.org/10.1016/j.xpro.2024.103020 | DOI Listing |
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