-Derived Extracellular Vesicles Prevent Hepatocellular Carcinoma by Modulating the TGF-β1/Smad Signaling in Mice.

Front Biosci (Landmark Ed)

Department of Pharmacology, School of Basic Medical Sciences, Jinzhou Medical University, 121001 Jinzhou, Liaoning, China.

Published: June 2024

Background: The involvement of gut microbiota in carcinogenesis has gradually been highlighted in past decades. Bacteria could play its role by the secretion of extracellular vesicles (EVs); however, interrelationship between bacterial EVs and hepatocellular carcinoma (HCC) development has not been investigated much.

Methods: Diethylnitrosamine (DEN) was utilized to produce HCC model in mice, of which fecal was collected for detecting () with real-time polymerase chain reaction (PCR). EV isolated from (-EV) with ultracentrifugation were stained with PKH26 to investigate the cellular uptake of murine hepatocytes (AML12). After treatment with -EV, TGF-β1-induced AML12 cells were subjected to morphological observation, fibrosis- and apoptosis-related marker detection with western blot, apoptotic ratio and reactive oxygen species (ROS) level analysis with flow cytometry, and oxidative stress biomarker assessment with enzyme-linked immunosorbent assay (ELISA); meanwhile, animal studies including liver function, tumor formation rate, and histological analysis, were also performed to investigate the role of -EV in the fibrosis, apoptosis, oxidative stress, and carcinogenesis of the liver .

Results: The levels of were significantly reduced in HCC model mice. -EV could enter AML12 cells and effectively attenuate TGF-β1-induced fibrosis, apoptosis, and oxidative stress in AML12 cells. studies showed that -EV administration alleviated DEN-induced liver fibrosis, apoptosis, and oxidative stress at the early stage. Moreover, -EV administration also effectively reduced the tumor formation rate and liver function injury in DEN-induced mice and down-regulated TGF-β1 expression and Smad3 phosphorylation of mouse liver.

Conclusions: -EVs protect hepatocytes against fibrosis, apoptosis, and oxidative damage, which exert a potential of preventing HCC development.

Download full-text PDF

Source
http://dx.doi.org/10.31083/j.fbl2907241DOI Listing

Publication Analysis

Top Keywords

oxidative stress
16
fibrosis apoptosis
16
apoptosis oxidative
16
aml12 cells
12
extracellular vesicles
8
hepatocellular carcinoma
8
hcc development
8
hcc model
8
model mice
8
liver function
8

Similar Publications

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!