AI Article Synopsis
- * In a study involving mouse skin transplants, GILs present at day 5 or day 7 post-transplant led to complete rejection of grafts, while GILs from day 3 did not induce rejection.
- * The findings suggest that GILs' ability to promote or prevent rejection is time-dependent, indicating that day 3 GILs had no effect on promoting graft rejection in skin transplant scenarios.
Article Abstract
Graft-infiltrating lymphocytes (GILs) play an important role in promoting rejection after organ transplantation. We recently reported that GILs that accumulated up to 3 days post-transplantation did not promote rejection, whereas GILs present 3-5 days post-transplantation promoted rejection in a mouse heart transplantation model. However, the immunological behaviour of GILs in murine skin transplantation remains unclear. GILs were isolated on days 3, 5 or 7 post-transplantation from C57BL/6 (B6) allogeneic skin grafts transplanted onto BALB/c mice. BALB/c Rag2 γc mice (BRGs) underwent B6 skin graft transplantation 10 weeks after adoptive transfer of day 3, 5, or 7 GILs. BRGs reconstituted with day 5 or 7 GILs completely rejected B6 grafts. However, when B6 grafts harvested from recipient BALB/c mice on day 5 or 7 were re-transplanted into BRGs, half of the re-transplanted day 5 grafts established long-term survival, although all re-transplanted day 7 grafts were rejected. BRGs reconstituted with day 3 GILs did not reject B6 grafts. Consistently, re-transplantation using day 3 skin grafts resulted in no rejection. Administration of anti-CD25 antibodies did not prevent the phenomenon observed for the day 3 skin grafts. Furthermore, BRGs reconstituted with splenocytes from naïve BALB/c mice immediately rejected the naïve B6 skin grafts and the re-transplanted day 3 B6 grafts, suggesting that day 3 GILs were unable to induce allograft rejection during the rejection process. In conclusion, the immunological role of GILs depends on the time since transplantation. Day 3 GILs had neither protective nor alloreactive effects in the skin transplant model.
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| http://dx.doi.org/10.1111/sji.13397 | DOI Listing |
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Article Synopsis
- * In a study involving mouse skin transplants, GILs present at day 5 or day 7 post-transplant led to complete rejection of grafts, while GILs from day 3 did not induce rejection.
- * The findings suggest that GILs' ability to promote or prevent rejection is time-dependent, indicating that day 3 GILs had no effect on promoting graft rejection in skin transplant scenarios.
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