AI Article Synopsis
- Periodontitis is a chronic inflammatory disease that leads to plaque buildup and damage to the bone that supports teeth, prompting the need for effective treatments to promote bone healing.
- Researchers developed a new biomaterial called C-KR8, which helps switch macrophages to a regenerative state and supports bone growth in conditions like periodontitis.
- C-KR8 is delivered using nanoparticles (C-KR8@ZIF-8) that release it and zinc; this approach enhances the healing process by interacting with key proteins and activating important cell signaling pathways.
Article Abstract
Periodontitis is a chronic inflammatory disease characterized by plaque accumulation, resulting in immune microenvironment disorders and resorption of alveolar bone. To promote bone healing under inflammatory environments, a functional biomaterial based on disease pathophysiology is designed. A novel fatty acid C10-modified polypeptide, C-KR8, is discovered to have excellent abilities in modulating macrophage repolarization and promoting bone regeneration in periodontitis. To build a multifunctional material localized drug delivery system, C-KR8@ZIF-8 (C-KR8-loaded zeolitic imidazolate framework-8) nanoparticles are constructed to sustainedly release the C-KR8 peptide and Zn elements. By synergistic effects of providing a dynamic immuno-modulatory environment and promoting osteogenesis under pathological conditions, the obtained pH-responsive nanoparticles display excellent bone regeneration capability. Furthermore, coimmunoprecipitation/liquid chromatography-tandem mass spectrometry analysis and proteomics analysis revealed that the C-KR8 peptide directly interacts with the high-temperature requirement protein A1 (Htra1), and C-KR8@ZIF-8 nanoparticles promote the osteogenic differentiation of bone mesenchymal stem cells by activating the focal adhesion kinase (FAK)/phosphatidylinositide 3-kinase (PI3K)/AKT pathway and enhancing the nuclear localization of Yes-associated protein (YAP). Taken together, this study demonstrates C-KR8 peptide regulate osteoimmunology and bone regeneration by Htra1/FAK/YAP pathway and that ZIF-8-based peptide loading platform is a promising strategy for periodontitis.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11283871 | PMC |
| http://dx.doi.org/10.34133/bmr.0057 | DOI Listing |
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