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Molecular characterisation of IBDV isolates in Turkey revealed reassortant strains. | LitMetric

Molecular characterisation of IBDV isolates in Turkey revealed reassortant strains.

Br Poult Sci

Faculty of Veterinary Medicine, Department of Microbiology, Ankara University, Ankara, Turkey.

Published: December 2024

AI Article Synopsis

  • Infectious bursal disease (IBD) is a highly contagious viral infection in chickens, caused by the IBD virus (IBDV), which has a complex RNA genome and five proteins.
  • Researchers examined samples from 50 chicken flocks in Turkey suspected of IBD, utilizing RT-PCR analysis to test for the disease.
  • The results revealed that 60% of the samples were positive for the VP2 gene, indicating the presence of reassortant strains causing the disease in broiler flocks throughout the country.

Article Abstract

1. Infectious bursal disease (IBD) is an acute, highly contagious viral disease of chickens caused by a virus (IBDV) which has a bi-segmented, double-stranded RNA genome. It has five viral proteins in its structure; the VP1 gene is encoded in segment B and the other four are in segment A.2. In this study, bursae of Fabricius and spleen samples taken from chickens suspected of having clinical or subclinical IBD from a total of 50 chicken flocks located in different geographical regions of Turkey were examined.3. The RT-PCR analysis of the VP2 gene showed that 30 of the 50 samples (60%) tested positive. Eight positive isolates were chosen and RT-PCR was performed to amplify the VP1 gene.4. The study showed that reassortant field strains that cause clinical or subclinical disease are currently circulating in broiler flocks across Turkey.

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Source
http://dx.doi.org/10.1080/00071668.2024.2379950DOI Listing

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