AI Article Synopsis
- The study compares the NeuMoDx testing method to the traditional artus qPCR method for detecting BKPyV in transplant patients by analyzing 95 samples, including both infected and control cases.
- Results show that both methods yield similar positivity rates and indicate a strong correlation, suggesting NeuMoDx is a reliable alternative.
- An equation for converting measurements between the two methods was provided, allowing for better monitoring of BKPyV infections in transplant patients.
Article Abstract
Purpose: The study aims to compare random-access NeuMoDx values with artus qPCR values to validate the accuracy of NeuMoDx as an alternative to qPCR and provide an equation to convert copies/ml to IU/ml measurements.
Methods: A total of 95 samples, including 61 transplant patient samples (n = 23 urine, n = 38 plasma) as the study group, 28 BKPyV-free samples as the control group, and six quality control samples, were included. One-Way ANOVA, Pearson correlation, Bland-Altman, Passing-Bablok, Deming regression analyses were used for statistical evaluation.
Results: Of 95 samples, 46 (48 %) were positive with NeuMoDx, while 40 (42 %) were positive with artus qPCR. Both techniques were statistically similar (p > 0.05). Deming correlation analysis (r = 0.9590), Passing Bablok and Bland Altman analyses demonstrated a strong correlation between NeuMoDx and artus values. The equation that provides the conversion between NeuMoDx and artus qPCR values was NeuMoDx= (1.12965 x artus qPCR) - 0.55016. BKPyV infections remain a concern for transplant patients globally, and effective new diagnostic methods are required.
Conclusions: Consistency between the results of NeuMoDx and qPCR confirms that NeuMoDx may be a valuable alternative for detecting BKPyV to prevent viral propagation. Our findings may allow converting copy/ml results to IU/ml for diagnosing and monitoring BKPyV infections in transplant patients.
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| http://dx.doi.org/10.1016/j.ijmmb.2024.100687 | DOI Listing |
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