AI Article Synopsis
- MRSA (methicillin-resistant Staphylococcus aureus) poses a significant health threat due to its ability to cause infections in both humans and animals, especially strains that are multidrug-resistant (MDR) and biofilm-producing like MTR_BAU_H1.
- The MTR_BAU_H1 strain was identified through hand swabs from food handlers in Bangladesh and underwent whole-genome sequencing to analyze its genetic traits, including antimicrobial resistance and virulence gene distribution.
- The study revealed that MTR_BAU_H1 belongs to sequence type ST1930 and contains numerous genes related to antimicrobial resistance and virulence, highlighting its potential impact on staphylococcal infections in people and animals.
Article Abstract
Methicillin-resistant (MRSA) is an important zoonotic pathogen associated with a wide range of infections in humans and animals. Thus, the emergence of MRSA clones poses an important threat to human and animal health. This study is aimed at elucidating the genomics insights of a strong biofilm-producing and multidrug-resistant (MDR) MTR_BAU_H1 strain through whole-genome sequencing (WGS). The MTR_BAU_H1 strain was isolated from food handlers' hand swabs in Bangladesh and phenotypically assessed for antimicrobial susceptibility and biofilm production assays. The isolate was further undergone to high throughput WGS and analysed using different bioinformatics tools to elucidate the genetic diversity, molecular epidemiology, sequence type (ST), antimicrobial resistance, and virulence gene distribution. Phenotypic analyses revealed that the MTR_BAU_H1 strain is a strong biofilm-former and carries both antimicrobial resistance (e.g., methicillin resistance; A, beta-lactam resistance; Z and tetracycline resistance; C) and virulence (e.g., , , and ) genes. The genome of the MTR_BAU_H1 belonged to ST1930 that possessed three plasmid replicons (e.g., rep16, rep7c, and rep19), seven prophages, and two clustered regularly interspaced short palindromic repeat (CRISPR) arrays of varying sizes. Phylogenetic analysis showed a close evolutionary relationship between the MTR_BAU_H1 genome and other MRSA clones of diverse hosts and demographics. The MTR_BAU_H1 genome harbours 42 antimicrobial resistance genes (ARGs), 128 virulence genes, and 273 SEED subsystems coding for the metabolism of amino acids, carbohydrates, proteins, cofactors, vitamins, minerals, and lipids. This is the first-ever WGS-based study of a strong biofilm-producing and MDR strain isolated from human hand swabs in Bangladesh that unveils new information on the resistomes (ARGs and correlated mechanisms) and virulence potentials that might be linked to staphylococcal pathogenesis in both humans and animals.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11283335 | PMC |
| http://dx.doi.org/10.1155/2024/5516117 | DOI Listing |
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