Protocol for collecting xylem sap from drought-treated tomato plants using a pressure chamber.

When plants are subjected to drought, it is impossible to obtain xylem sap for subsequent biochemical and molecular analysis using root pressure exudate, the conventional approach. Here, we present a protocol for collecting xylem sap from drought-treated tomato plants using a pressure chamber. We describe steps for how to prepare plants, apply drought, and use the pressure chamber to collect the xylem sap. Using this technique, one can obtain 500-700 μL of xylem sap in just 5-7 min. For complete details on the use and execution of this protocol, please refer to Alexou and Peuke..