AI Article Synopsis
- - Centromere length changes during cell division can be assessed by measuring the copy numbers of higher-order repeats (HORs), which are essential parts of centromeric regions.
- - The study introduces a method for isolating single cells and using droplet digital PCR to quantify HOR CNs in different subclones, helping to track changes over time.
- - The protocol aims to explore the molecular mechanisms behind the quick evolution of centromere sequences, with further details available in the work of Showman et al.
Article Abstract
Centromere length changes occurring during somatic cell divisions can be estimated by quantifying the copy numbers (CNs) of higher-order repeats (HORs), which are nested repeats of monomers that comprise centromeric arrays. Here, we present a protocol for single-cell isolation for clonal evolution followed by droplet digital PCR-based quantification. The assay measures HOR CNs across subclones to determine the frequency and degree of changes in HOR CNs. This protocol tests the underlying molecular mechanisms responsible for rapid centromere sequence evolution. For complete details on the use and execution of this protocol, please refer to Showman et al..
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11338184 | PMC |
| http://dx.doi.org/10.1016/j.xpro.2024.103218 | DOI Listing |
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