Introduction: Oral squamous cell carcinoma (OSCC) includes about 90% of all oral malignant tumors, and most of them are diagnosed in advanced stages. This study investigated the expression changes of miR-24, miR-200, and miR-34 in saliva samples of patients with oral squamous cell carcinoma, for early diagnosis.
Methods: In this study, 30 patients and 30 healthy individuals were selected. After RNA extraction and cDNA synthesis, the expression levels of miR-24, miR-200, and miR-34 in saliva samples were measured and evaluated using the Real-Time PCR technique.
Results: Folding change calculation using 2^(-∆∆ Ct) refers to the relative difference in the expression of the markers of the two groups. The expression level of two biomarkers, miR-200 and miR-34, is decreased in patients compared to healthy people; and the expression level of miR-24 is increased in patients compared to healthy people.
Conclusion: In general, considering the availability and convenience of saliva sample collection for early detection of the disease, this research result can be considered a diagnostic screening test. To further prove the research results, conducting more extensive studies with more samples is recommended.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11480605 | PMC |
| http://dx.doi.org/10.31557/APJCP.2024.25.7.2265 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Genetic and epigenetic regulation of non-coding RNAs: Implications in cancer metastasis, stemness and drug resistance.
Pathol Res Pract
November 2024
Institute of Bioinformatics, International Technology Park, Bangalore 560066, India; Manipal Academy of Higher Education (MAHE), Manipal, Karnataka 576104, India. Electronic address:
Article Synopsis
- * Specific ncRNAs regulate crucial signaling pathways (e.g., Wnt, Notch, Hedgehog) that affect CSC traits like self-renewal and differentiation, with some microRNAs inhibiting these processes while certain lncRNAs enhance stemness.
- * Understanding the interplay between ncRNAs and CSCs can lead to innovative targeted therapies that address aggressive and treatment-resistant cancers, providing new avenues for effective cancer treatment.
Inhalation Exposure to Airborne PM Attenuates Hepatic Metabolic Pathways through S-Nitrosylation of the Primary ER Stress Sensor.
Am J Physiol Cell Physiol
November 2024
From Center for Molecular Medicine & Genetics, Department of Biochemistry, Microbiology, and Immunology, The Wayne State University School of Medicine, Detroit, MI 48201, USA.
Inhalation exposure to airborne fine particulate matter (aerodynamic diameter < 2.5 µm, PM) is known to cause metabolic dysfunction-associated steatohepatitis (MASH) and the associated metabolic syndrome. Hepatic lipid accumulation and inflammation are the key characteristics of MASH.
View Article and Find Full Text PDFBiomarkers for Oral Squamous Cell Carcinoma (miR-24, miR-200, and miR-34): Screening and Detection MicroRNA.
Asian Pac J Cancer Prev
July 2024
Chronic Respiratory Diseases Research Center, NRITLD, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Introduction: Oral squamous cell carcinoma (OSCC) includes about 90% of all oral malignant tumors, and most of them are diagnosed in advanced stages. This study investigated the expression changes of miR-24, miR-200, and miR-34 in saliva samples of patients with oral squamous cell carcinoma, for early diagnosis.
Methods: In this study, 30 patients and 30 healthy individuals were selected.
Low levels of miR-143-3p are associated with severe chronic rhinosinusitis with nasal polyps.
Rhinology
August 2024
INGENIO, Clinical and Experimental Respiratory Immunoallergy (IRCE), Institut d'Investigacions Biomediques August Pi i Sunyer (IDIBAPS), Barcelona, Catalonia, Spain; CIBER of Respiratory Diseases (CIBERES), Carlos III Health Institute, Madrid, Spain; Universitat de Barcelona. Barcelona, Catalonia, Spain;CIBER of Respiratory Diseases (CIBERES), Carlos III Health Institute, Madrid, Spain;Rhinology Unit and Smell Clinic, ENT Department, Hospital Clinic Barcelona, Universitat de Barcelona, Barcel.
microRNAs (miRNAs) are small, single-stranded, non-coding RNA molecules that regulate post-transcriptional gene expression. Accumulating evidence suggests their involvement in regulating various biological and pathological processes, including inflammation. Studies have revealed distinct expression patterns of miRNAs in Chronic Rhinosinusitis with (CRSwNP) and without (CRSsNP) nasal polyps (1).
View Article and Find Full Text PDFThe role of flavonoids in the regulation of epithelial-mesenchymal transition in cancer: A review on targeting signaling pathways and metastasis.
Med Res Rev
November 2023
LAQV, REQUIMTE, Laboratory of Applied Chemistry, Department of Chemical Sciences, Faculty of Pharmacy, University of Porto, Porto, Portugal.
One of the hallmarks of cancer is metastasis, a process that entails the spread of cancer cells to distant regions in the body, culminating in tumor formation in secondary organs. Importantly, the proinflammatory environment surrounding cancer cells further contributes to cancer cell transformation and extracellular matrix destruction. During metastasis, front-rear polarity and emergence of migratory and invasive features are manifestations of epithelial-mesenchymal transition (EMT).
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!