AI Article Synopsis
- Lung neuroendocrine neoplasms (NENs) include rare tumors like lung carcinoid (LC), which show unique characteristics and are influenced by transcription factors (TFs).
- Recent research has identified a specific transcription factor, Prospero homeobox protein1 (PROX1), as highly expressed in LC tissues and suggested it might contribute to the tumor's neuroendocrine features.
- Although knocking down PROX1 did not affect cell viability in a specific LC cell line, it did change the expression of genes linked to NE differentiation, indicating PROX1's potential regulatory role in maintaining the characteristics of lung carcinoid tumors.
Article Abstract
Lung neuroendocrine neoplasms (NENs) are a diverse group of tumors characterized by neuroendocrine (NE) differentiation. Among lung NENs, lung carcinoid (LC) is a rare tumor with unique characteristics. Recent research has highlighted the importance of transcription factors (TFs) in establishing gene expression programs in lung NENs such as small cell lung carcinoma. However, the TFs that control the gene expression of LC are largely unknown. In this study, we report the expression and potential function of a TF called Prospero homeobox protein1 (PROX1) in LC. Publicly available transcriptome data suggested that PROX1 was highly expressed in LC tissues, which was confirmed by immunohistochemical analysis on a tissue microarray. Knockdown of PROX1 did not impact the cellular viability of an LC-derived cell line, NCI-H727. Meanwhile, transcriptome analysis revealed that PROX1 knockdown altered the expression of genes involved in NE differentiation. ASCL1, CHGA, CALCA, and LINC00261 were suggested as downstream genes of PROX1. These findings indicate that PROX1 may play an important role in the NE identity of LC by regulating the expression of key target genes.
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| http://dx.doi.org/10.1007/s13577-024-01109-3 | DOI Listing |
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