The Influence of Technological Factors on the Structure and Chemical Composition of Tuberous Roots Determined Using Vibrational Spectroscopy.

This research investigated the structural and chemical modifications of 'Kennemerland' across different technological conditions and throughout the vegetation period. Using FT-IR imaging, this study focused on the changes in the inulin, lignin, and suberin contents of tuberous roots. FT-IR maps were generated to visualize the distribution of these compounds across scanned areas, highlighting variations across cultivation methods and seasonal stages. The key compounds analyzed included inulin, lignin, and suberin, which were identified in different root zones. The results showed that inulin was distributed in all analyzed areas, predominantly in zone 1 (periderm), with a distribution that increased with forced cultivation, while lignin and suberin distributions varied with zone and season. Forced tuberous root lignin was detected in all four areas analyzed, in the fall accumulating mainly in area 4 and in suberin starting from summer until autumn. Based on the evaluation of the maps obtained by representing the area ratios of specific bands (inulin/lignin and inulin/suberin), we established where the inulin was present in the highest quantity and concluded that suberin was the constituent with the lowest concentration in tuberous roots. These findings emphasize the influence of technological factors and seasonal changes on the biochemical makeup of tuberous roots. This detailed biochemical mapping provides insights for optimizing cultivation and storage for various industrial applications. This study concludes that FT-IR spectroscopy is an effective tool for monitoring and understanding the biochemical dynamics of roots, aiding their agricultural and industrial utilization.