AI Article Synopsis
- Alternative polyadenylation (APA) significantly influences gene expression in eukaryotes, impacting processes like cell growth and differentiation, but its role during metamorphosis remains unclear.
- By analyzing RNA-seq data from embryos to maturation over 16 time points, researchers found 247 differentially expressed APA events and identified five distinct patterns, with the gradual elongation of the 3' UTR being the most prominent.
- The study highlights complex APA mechanisms during metamorphosis, revealing a connection between these changes and pathways related to protein and energy metabolism, along with potential regulatory roles of specific miRNAs.
Article Abstract
As an important mechanism in the post-transcriptional regulation of eukaryotic gene expression, alternative polyadenylation (APA) plays a key role in biological processes such as cell proliferation and differentiation. However, the role and dynamic pattern of APA during metamorphosis are poorly understood. Here, RNA-seq data covering from the embryo to the maturation (16 time points) of were utilized. We identified 247 differentially expressed APA events between early and adult stages, and through fuzzy mean clustering analysis, we discovered five dynamic APA patterns. Among them, the gradual elongation of the 3'UTR is the major APA pattern that changes over time, and its genes are enriched in the pathways of protein and energy metabolism. Finally, we constructed mRNA-miRNA and PPI networks and detected several central miRNAs that may regulate development. Our results revealed the complex APA mechanisms in metamorphosis, shedding new light on post-transcriptional regulation of crustacean metamorphosis.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11275414 | PMC |
| http://dx.doi.org/10.3390/genes15070837 | DOI Listing |
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