AI Article Synopsis
- * C57BL/6 mice were divided into groups for I/R injury, Daprodustat treatment before I/R, and a control group, employing various scientific methods to measure the effects of the treatment.
- * Results indicated that Daprodustat reduced cell death, oxidative stress, and inflammation, suggesting it could be a promising treatment for I/R-related muscle injuries.
Article Abstract
Purpose: The purpose of the present work was to assess the specific effects and underlying mechanisms of Daprodustat (GSK1278863) on skeletal muscle injury induced by ischemia reperfusion (I/R).
Methods: C57BL/6 mice were randomized into the skeletal muscle I/R injury (I/R), Daprodustat (GSK1278863) pretreatment and I/R (I/R + GSK) and sham operation (Sham) groups. The skeletal muscle I/R injury model was established by placing an orthodontic rubber band at the left hip joint for 3 h and releasing it for 3 h. H&E staining, wet weight/dry weight ratio assessment, TUNEL assay, ELISA, qRT-PCR and immunoblot were utilized to assess the effects of Daprodustat.
Results: Daprodustat pretreatment significantly ameliorated apoptosis in skeletal muscle cells, reduced oxidative damage and suppressed inflammatory cytokines. Mechanistically, Daprodustat positively affected NF-κB signaling activation.
Conclusion: These data demonstrated that Daprodustat may provide a potential clinical approach for preventing or treating skeletal muscle injury induced by I/R.
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| http://dx.doi.org/10.1177/10225536241267725 | DOI Listing |
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