AI Article Synopsis
- - The combination of DNA origami and surface-enhanced Raman spectroscopy (SERS) has led to significant advancements in detecting single-molecule proteins, improving biomedical diagnostics.
- - By using DNA origami, researchers have created gold nanorod structures for enhanced SERS signal, allowing for better detection of proteins.
- - The study specifically focused on the epidermal growth factor receptor (EGFR), showing that gold nanorod dimer assemblies can effectively identify single protein signals, which is crucial for cancer research.
Article Abstract
The convergence of DNA origami and surface-enhanced Raman spectroscopy (SERS) has opened a new avenue in bioanalytical sciences, particularly in the detection of single-molecule proteins. This breakthrough has enabled the development of advanced sensor technologies for diagnostics. DNA origami offers a highly controllable framework for the precise positioning of nanostructures, resulting in superior SERS signal amplification. In our investigation, we have successfully designed and synthesized DNA origami-based gold nanorod monomer and dimer assemblies. Moreover, we have evaluated the potential of dimer assemblies for label-free detection of a single biomolecule, namely epidermal growth factor receptor (EGFR), a crucial biomarker in cancer research. Our findings have revealed that the significant Raman amplification generated by DNA origami-assembled gold nanorod dimer nanoantennas facilitates the label-free identification of Raman peaks of single proteins, which is a prime aim in biomedical diagnostics. The present work represents a significant advancement in leveraging plasmonic nanoantennas to realize single protein SERS for the detection of various cancer biomarkers with single-molecule sensitivity.
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| http://dx.doi.org/10.1039/d4nr01110d | DOI Listing |
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