AI Article Synopsis
- The study investigates the role of the extracellular matrix (ECM) in uterine tube physiology and aims to create region-specific decellularized scaffolds from porcine uterine tubes for tissue engineering applications.
- To achieve this, porcine uterine tubes were treated with specific solutions to remove cellular components while preserving the ECM structure and composition.
- Experimental results showed successful decellularization, maintaining the scaffold’s structural integrity, and high cell viability, indicating potential use in reproductive biotechnology.
Article Abstract
The uterine tube extracellular matrix is a key component that regulates tubal tissue physiology, and it has a region-specific structural distribution, which is directly associated to its functions. Considering this, the application of biological matrices in culture systems is an interesting strategy to develop biomimetic tubal microenvironments and enhance their complexity. However, there are no established protocols to produce tubal biological matrices that consider the organ morphophysiology for such applications. Therefore, this study aimed to establish region-specific protocols to obtain decellularized scaffolds derived from porcine infundibulum, ampulla, and isthmus to provide suitable sources of biomaterials for tissue-engineering approaches. Porcine uterine tubes were decellularized in solutions of 0.1% SDS and 0.5% Triton X-100. The decellularization efficiency was evaluated by DAPI staining and DNA quantification. We analyzed the ECM composition and structure by optical and scanning electronic microscopy, FTIR, and Raman spectroscopy. DNA and DAPI assays validated the decellularization, presenting a significative reduction in cellular content. Structural and spectroscopy analyses revealed that the produced scaffolds remained well structured and with the ECM composition preserved. YS and HEK293 cells were used to attest cytocompatibility, allowing high cell viability rates and successful interaction with the scaffolds. These results suggest that such matrices are applicable for future biotechnological approaches in the reproductive field.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11274565 | PMC |
| http://dx.doi.org/10.3390/biomimetics9070382 | DOI Listing |
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