AI Article Synopsis
- This study explores how non-ionic surfactants, specifically Cremophor EL and Cremophor RH40, can help inhibit efflux pumps in multidrug-resistant (MDR) bacteria, which often render antibiotics ineffective.
- Researchers confirmed the presence of MDR strains and tested how these surfactants, combined with ciprofloxacin, affected minimum inhibitory concentrations (MIC).
- Results showed that both surfactants significantly reduced MIC values of ciprofloxacin, indicating their potential to enhance the effectiveness of existing antibiotics against resistant strains.
Article Abstract
Background: There is increasing emphasis on restoring the efficacy of existing antibiotics instead of developing new ones.
Objectives: This study aimed to determine the role of Cremophor EL and Cremophor RH40 in the inhibition of efflux pumps in MDR strains.
Methods: Efflux pump-active MDR strains of were identified and confirmed by flow cytometry. The identified efflux-active strains were further subjected to determination of the MIC of ciprofloxacin and the synergistic role of non-ionic surfactants (Cremophor EL and Cremophor RH40) along with ciprofloxacin.
Results: Out of 30 samples, 6 strains displayed high efflux pump activity. Both Cremophor EL and Cremophor RH40 showed efflux pump inhibitory roles. A 4-fold reduction in the MIC values of ciprofloxacin was observed when Cremophor EL was used along with ciprofloxacin, while a 6-fold reduction was observed when Cremophor RH40 was used along with ciprofloxacin. Both compounds showed synergistic effects with ciprofloxacin, ticarcillin and meropenem when used in a 24-well plate efflux pump inhibitory assay.
Conclusion: The inhibition of the efflux pump of MDR by non-ionic surfactants, namely, Cremophor RH40 and Cremophor EL, provided the best strategy to restore the efficacy of ciprofloxacin.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11269872 | PMC |
| http://dx.doi.org/10.1016/j.heliyon.2024.e33749 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!