Mucins secreted by mucous cells constitute a core part of the defense line against the invasion of pathogens. However, mucins' structure and immunological functions remain largely unknown in teleost fish. In this study, two typical mucins, Muc2 and Muc5ac of flounder (Paralichthys olivaceus), were cloned and their physicochemical properties, structure and conservation were analyzed. Notably, specific antibodies against flounder Muc2 and Muc5ac were developed. It was verified at the gene and protein level that Muc2 was expressed in the hindgut and gills but not in the skin, while Muc5ac was expressed in the skin and gills but not in the hindgut. After flounders were immunized by immersion with inactivated Edwardsiella tarda, Muc2 and Muc5ac were significantly upregulated at both the gene expression and protein levels, and Muc2/Muc5ac mucous cells proliferated and increased secretion of Muc2 and Muc5ac. Moreover, Muc2 and Muc5ac exerted retention and clearance effects on E. tarda in a short period (within 1 dpi). These results revealed the characterization of fish mucins Muc2 and Muc5ac at the protein level and clarified the role of mucins as key guardians to maintain the mucus barrier, which advanced our understanding of teleost mucosal barrier.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.ijbiomac.2024.134127 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Effects of on-farm hatching versus hatchery hatching on growth performance, gut development, and intestinal health and function in broiler chickens.
Poult Sci
January 2025
Nutrition and Animal-Microbiota Ecosystems Laboratory, Department of Biosystems, KU Leuven 3000, Heverlee, Belgium. Electronic address:
An alternative hatching system known as hatch on-farm (HOF) provides early access to feed compared to hatch in hatchery (HH) system. Early feeding may promote favorable gut development, potentially improving intestinal health and broiler performance. Previous studies have assessed the effects of HOF on chick quality, welfare and performance, its impacts on gut health remain inconclusive.
View Article and Find Full Text PDFExploring the effects of lactulose on lung-intestinal tissue-associated factors and the TLR4/NF-κB signaling pathway in COPD rats based on lung-gut axis theory.
Microb Pathog
December 2024
Baotou Medical College, Inner Mongolia University of Science and Technology, Baotou, 014000, China; Department of Gastroenterology, The First Affiliated Hospital of Baotou Medical College, Inner Mongolia University of Science and Technology, Baotou, 014000, China. Electronic address:
Chronic obstructive pulmonary disease (COPD) is a systemic inflammatory disease impacting both the respiratory and gastrointestinal systems, with its pathogenesis closely linked to the lung-gut axis theory. In this study, we established a rat model of COPD using a fumigation method combined with intra-airway administration of lipopolysaccharide (LPS) to investigate the effects of lactulose on lung and intestinal tissues, focusing on related inflammatory markers and the TLR4/NF-κB signaling pathway. We further explored the therapeutic effects and mechanisms of lactulose on the lung-intestinal tissues in COPD rats, aiming to expand its potential application in chronic respiratory diseases.
View Article and Find Full Text PDFClinically relevant cell culture model of inflammatory bowel diseases for identification of new therapeutic approaches.
Int J Pharm
January 2025
Université de Franche-Comté, EFS, INSERM, UMR RIGHT, LabEx LipSTIC (ANR-11- LABX-0021), F-25000 Besançon, France. Electronic address:
Inflammatory Bowel Diseases (IDB) are chronic disorders characterized by gut inflammation, mucosal damage, increased epithelial permeability and altered mucus layer. No accurate in vitro model exists to simulate these characteristics. In this context, drug development for IBD or intestinal inflammation requires in vivo evaluations to verify treatments efficacy.
View Article and Find Full Text PDFEsophageal submucosal gland duct adenoma: An unrecognised esophageal counterpart of minor salivary gland tumours with frequent BRAF V600E mutations.
Histopathology
December 2024
Department of Pathology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, China.
Aims: Esophageal submucosal gland duct adenoma is an extremely rare benign tumour, with only a few reported cases. We conducted the largest single-centre clinical study of esophageal submucosal gland duct adenoma, examining its molecular mechanisms and clinicopathological features.
Methods And Results: Between 2018 and 2023, seven cases of esophageal submucosal gland duct adenoma were identified at a tertiary medical centre; two were female and five were male, aged between 51 and 75 years (mean = 63.
Deciphering the toxicity of polyhexamethylene guanidine phosphate in lung carcinogenesis: Mutational profiles and molecular mechanisms.
Chemosphere
November 2024
Department of Pathology, Korea University Ansan Hospital, Korea University College of Medicine, Ansan, Gyeonggi, 15355, Republic of Korea. Electronic address:
Article Synopsis
- - Polyhexamethylene guanidine phosphate (PHMG-p) is commonly found in personal hygiene products but its long-term health effects, specifically regarding lung damage and cancer from respiratory exposure, are not well understood.
- - The study investigated the kinds of lung lesions and cancer risk from PHMG-p through CT scans and pathology over 54 weeks, revealing that PHMG-p is linked to mutations in key cancer-related genes and disruptions in DNA repair pathways.
- - Molecular analyses showed PHMG-p promotes necroptosis and activates certain signaling pathways while inhibiting others related to immune responses, suggesting a mechanism for how PHMG-p exposure leads to lung-related health issues and potential cancer development.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!