AI Article Synopsis
- * Researchers analyzed 146 isolates, identifying various mutations and assessing their impact on EMB resistance through minimum inhibitory concentration testing and statistical modeling.
- * Results showed that certain mutations (Met306Val, Met306Ile, Gly406Ala, and Gln497Arg) were significantly related to EMB resistance, with some mutations strongly correlating with high-level resistance, highlighting the complex genetic factors influencing EMB susceptibility.
Article Abstract
Objective: To explore the association between the variant mutations within and , and the degree of ethambutol (EMB) resistance of () isolates.
Methods: A total of 146 isolates were used to determine the minimum inhibitory concentrations (MICs) of EMB with a 96-well microplate-based assay. The mutations within and among these isolates were identified with DNA sequencing. Moreover, a multivariate regression model and a computer model were established to assess the effects of mutations on EMB resistance.
Results: Our data showed that overall 100 isolates exhibited 28 mutated patterns within the sequenced and . Statistical analysis indicated that mutations Met306Val, Met306Ile, Gly406Ala, and Gln497Arg, were strongly associated with EMB resistance. Of these mutations, Met306Val and Gln497Arg were significantly associated with high-level EMB resistance. Almost all multiple mutations occurred in high-level EMB-resistant isolates. Although the mutation within accompanied with mutation presented exclusively in EMB-resistant isolates, four single mutations (Ala39Glu, Ser173Ala, Trp175Cys, and Val283Leu) leading to protein instability were observed in EMB-susceptible isolates.
Conclusion: This study highlighted the complexity of EMB resistance. Some individual mutations and multiple mutations within and contributed to the different levels of EMB resistance.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11268779 | PMC |
| http://dx.doi.org/10.2147/IDR.S466371 | DOI Listing |
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