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Molecular identification of polymorphic transposable elements in populations of the invasive ant . | LitMetric

Molecular identification of polymorphic transposable elements in populations of the invasive ant .

Biol Methods Protoc

Institute for Evolution and Biodiversity, University of Münster, Hüfferstraße 1, Münster 48149, Germany.

Published: July 2024

AI Article Synopsis

  • * The Transposon Display (TD) technique enables the identification of genetic polymorphisms by amplifying specific TEs, primarily used in plants, but now optimized for small-genome insects like ants.
  • * This study used the TD protocol to analyze TE polymorphisms in invasive ant lineages, discovering active retrotransposons that contribute to genetic diversity within populations.

Article Abstract

Transposable elements (TEs) are found in virtually every eukaryotic genome and are important for generating genetic variation. However, outside of costly and time-consuming whole-genome sequencing approaches, the set of available methods to study TE polymorphisms in non-model species is very limited. The Transposon Display (TD) is a simple yet effective technique to characterize polymorphisms across samples by identifying amplified fragment length polymorphisms using primers targeting specific TE families. So far, this technique has almost exclusively been used in plants. Here, we present an optimized TD protocol for insect species with small genomes such as ants (ca. 200-600 Mb). We characterized TE polymorphisms between two distinct genetic lineages of the invasive ant , as well as between neighboring populations of the New World lineage. We found active LTR/Ty3 retrotransposons, that contributed to the genetic diversification of populations in this species.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11268152PMC
http://dx.doi.org/10.1093/biomethods/bpae050DOI Listing

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