Straw cellulose is an abundant renewable resource in nature. In recent years, the conversion of cellulose from waste straw into biofuel by specific microorganisms' fragmentation has attracted extensive attention. Although many bacteria with the ability to degrade cellulose have been identified, comprehensive bioinformatics analyses of these bacteria remain limited, and research exploring optimal fragmentation conditions is scarce. Our study involved the isolation and screening of bacteria from various locations in Yangzhou using carboxymethyl cellulose (CMC) media. Then, the cellulose-degrading bacteria were identified using 16S rRNA and seven candidate bacterial strains with cellulose degrading ability were identified in Yangzhou city for the first time. The cellulase activity was determined by the 3,5-dinitrosalicylic acid (DNS) method in different fragmentation conditions, and finally two bacteria strains with the strongest cellulose degradation ability were selected for whole genome sequencing analysis. Sequencing results revealed that the genome sizes of YZ02 and YZ03 were 8.51 Mb and 6.66 Mb, containing 8,466 and 5,745 genes, respectively. A large number of cellulose degradation-related genes were identified and annotated using KEGG, GO and COG analyses. In addition, genomic CAZyme analysis indicated that both YZ02 and YZ03 harbor a series of glycoside hydrolase family (GH) genes and other genes related to cellulose degradation. Our finding provides new options for the development of cellulose-degrading bacteria and a theoretical basis for improving the cellulose utilization of straw.
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http://dx.doi.org/10.3389/fmicb.2024.1409697 | DOI Listing |
Huan Jing Ke Xue
January 2025
College of Environmental Science and Engineering, Hebei University of Science and Technology, Shijiazhuang 050018, China.
Straw incorporation can improve soil fertility and soil structure. While numerous studies have explored the immediate impacts of straw return on soil properties and crop production, the legacy effects of long-term straw return remain less understood. In this study, the straw returning soil of a continuous 15 years (SS) and non-straw returning soil (NS) were collected from Dahe Experimental Station of Hebei Academy of Agriculture and Forestry Sciences in China.
View Article and Find Full Text PDFFront Microbiol
December 2024
College of Food Science and Engineering, Inner Mongolia Agricultural University, Hohhot, China.
The enhancement of cellulose degradation is important for improving the quality of corn-stalk silage. However, the rapid drop in pH caused by the propagation of lactic acid bacteria (LAB) can influence the degradation of cellulose by cellulose-degrading microorganisms (CDMs) during the mixed fermentation process of ensilage. In this study, a CDM ( 2-4, BM 2-4) was isolated, and its lyophilization condition was studied.
View Article and Find Full Text PDFPest Manag Sci
December 2024
Yangtze Delta Region Institute (Quzhou), University of Electronic Science and Technology of China, Quzhou, China.
Appl Environ Microbiol
December 2024
Faculty of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences (NMBU), Ås, Norway.
Unlabelled: Freshwater ecosystems can be largely affected by neighboring agriculture fields where potential fertilizer nitrate run-off may leach into surrounding water bodies. To counteract this eutrophic driver, farmers in certain areas are utilizing denitrifying woodchip bioreactors (WBRs) in which a consortium of microorganisms convert the nitrate into nitrogen gases in anoxia, fueled by the degradation of lignocellulose. Polysaccharide-degrading strategies have been well described for various aerobic and anaerobic systems, including the use of carbohydrate-active enzymes, utilization of lytic polysaccharide monooxygenases (LPMOs) and other redox enzymes, as well as the use of cellulosomes and polysaccharide utilization loci (PULs).
View Article and Find Full Text PDFEnviron Res
January 2025
College of Ecology, Northeast Forestry University, Harbin, 150040, China; Key Laboratory of Sustainable Forest Ecosystem Management-Ministry of Education, Northeast Forestry University, Harbin, 150040, China; Northeast Asia Biodiversity Research Center, Northeast Forestry University, Harbin, 150040, China. Electronic address:
The purpose of this paper was to discover the key factors driving the lignocellulose degradation in litter along a forest succession chronosequence from the perspective of functional genes. We investigated four natural successive stages of forests (white birch forest, broad-leaved mixed forest, coniferous-broad-leaved mixed forest, and mixed broadleaved-Korean pine forest). We determined the lignocellulose degradation of litter and the absolute abundance of related functional genes by using high-throughput-qPCR.
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