AI Article Synopsis

  • * Traditional methods for isolating these cells often use fluorescent markers, which can complicate further analyses.
  • * The new Isosceles Trapezoidal Spiral Microchannel (ITSμC) effectively separates PGCCs from other cancer cells without needing fluorescent labeling, achieving high purity and viability rates (over 90%).

Article Abstract

Polyploid giant cancer cells (PGCCs) contribute to the genetic heterogeneity and evolutionary dynamics of tumors. Their size, however, complicates their isolation from mainstream tumor cell populations. Standard techniques like fluorescence-activated cell sorting (FACS) rely on fluorescent labeling, introducing potential challenges in subsequent PGCC analyses. In response, we developed the Isosceles Trapezoidal Spiral Microchannel (ITSμC), a microfluidic device optimizing the Dean drag force () and exploiting uniform vortices for enhanced separation. Numerical simulations highlighted ITSμC's advantage in producing robust compared to rectangular and standard trapezoidal channels. Empirical results confirmed its ability to segregate larger polystyrene (PS) particles (avg. diameter: 50 μm) toward the inner wall, while directing smaller ones (avg. diameter: 23 μm) outward. Utilizing ITSμC, we efficiently isolated PGCCs from doxorubicin-resistant triple-negative breast cancer (DOXR-TNBC) and patient-derived cancer (PDC) cells, achieving outstanding purity, yield, and viability rates (all greater than 90%). This precision was accomplished without fluorescent markers, and the versatility of ITSμC suggests its potential in differentiating a wide range of heterogeneous cell populations.

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Source
http://dx.doi.org/10.1039/d4an00750fDOI Listing

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