AI Article Synopsis
- The infection affects about 50% of people globally, leading to chronic gastritis and gastric cancer, and resistance to antibiotics like clarithromycin complicates treatment efforts.
- A new method called SHIELD was developed for simultaneously detecting the infection and clarithromycin resistance, utilizing RAA and CRISPR/Cas13a technology for enhanced accuracy.
- SHIELD demonstrated high sensitivity and specificity, successfully diagnosing the infection and resistance mutations in saliva samples, suggesting its potential for rapid and large-scale clinical application.
Article Abstract
Background: Infection caused by () affects approximately 50% of the global population. It is a major pathogenic factor for chronic gastritis and gastric cancer. Besides, the resistance to antibiotics such as clarithromycin could reduce the eradication rate. Currently, there is an urgent need for a swift, easy to perform, and highly sensitive detection method for and clarithromycin resistance.
Methods: We used FAM/Digoxin labeled primers to amplify specific 23S rRNA fragments by Recombinase Aided Amplification (RAA), and resistance mutations were distinguished using CRISPR/Cas13a system combined with lateral flow strip. Twenty-eight saliva samples were analyzed using qPCR, gene sequencing and this method to evaluate the detection efficiency.
Results: We developed a simultaneous detection method for and clarithromycin resistance mutations named sensitive easy-read dual detection (SHIELD). The results showed both A2142G and A2143G mutant DNAs causing clarithromycin resistance could be distinguished from the wild type with a concentration of 50 copies/μL, and no cross-reaction with other 5 common gastrointestinal bacteria was observed. For the detection of in 28 saliva samples, the positive predictive value of this method was 100% (19/19) in comparison with qPCR. For detecting clarithromycin resistance, the positive predictive value of this method was 84.6% (11/13) compared with gene sequencing.
Conclusion: SHIELD assay showed high sensitivity and specificity in detecting and clarithromycin resistance mutations. It could be a potential measure in the rapid detection of , large-scale screening and guiding clinical medication.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11265216 | PMC |
| http://dx.doi.org/10.2147/IDR.S462963 | DOI Listing |
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