AI Article Synopsis
- The study addresses the challenge of identifying diagnostic markers for rheumatoid arthritis (RA) by isolating exosomes from serum samples of RA patients with varying disease activities and treatment statuses.
- RNA from these exosomes was analyzed through whole-transcriptome sequencing, focusing particularly on long non-coding RNAs (lncRNAs).
- Two lncRNAs, one up-regulated and one down-regulated, were identified as potential biomarkers for RA diagnosis, suggesting a significant role for serum exosome lncRNAs in the disease.
Article Abstract
The lack of diagnostic markers limits the window of effectiveness for rheumatoid arthritis (RA) therapies. Here, we isolated exosomes of serum samples from four distinct groups RA patients, according to disease activity and with/without medication. Then, total RNA of exosomes was extracted for whole-transcriptome sequencing. Focusing on lncRNA sequencing, gene ontology (GO) and kyoto encyclopedia of genes and genomes (KEGG) pathway enrichment analyses were performed. We found that the number of upregulated lncRNAs were significantly higher than that of downregulated lncRNAs in each four RA groups. And most importantly, we identified two specific lncRNAs from differentially expressed lncRNAs, TCONS_I2_00013502 (up-regulated) and ENST00000363624 (down-regulated) in RA. Receiver Operating Characteristic (ROC) curve analysis showed that the two lncRNAs were promising biomarkers for RA diagnosis. These findings highlight lncRNAs of the serum exosome are important biomarkers and provide application potential for diagnosis of RA.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11263027 | PMC |
| http://dx.doi.org/10.3389/fimmu.2024.1419683 | DOI Listing |
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