AI Article Synopsis
- Researchers developed a colloidal gold immunochromatographic test strip for quick detection of enramycin in feed using a specific monoclonal antibody.
- The test strip was optimized with specific concentrations of potassium carbonate, antibodies, and antigens, achieving good specificity and a low detection limit.
- It successfully detected enramycin A at 25 ng/mL, proving to be more sensitive and repeatable than traditional methods like high-performance liquid chromatography.
Article Abstract
To achieve rapid detection of enramycin in feed, we employed the competitive inhibition method to develop a colloidal gold immunochromatographic test strip based on the anti-enramycin A monoclonal antibody (anti-Er.A-mAb). Colloidal gold probes were prepared with a laboratory-prepared high-purity anti-Er.A-mAb. The effects of pH, antibody titer, and antigen concentration (test line) on the test strip performance were investigated. The colloidal gold test strip prepared with 8 μL potassium carbonate addition, 4 µg/mL antibody, 1.0 mg/mL antigen (test line), and 3 μL gold-labeled antibody showed acceptable specificity and a low limit of detection. The test strip showed the detection limit of 25 ng/mL for enramycin A, with a linear range of 25-300 ng/mL. The experiments on the feed with positive sample addition proved that the test strip had good repeatability and was more sensitive than high-performance liquid chromatography, being applicable for the rapid detection of enramycin in large batches of feed samples.
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| http://dx.doi.org/10.13345/j.cjb.230845 | DOI Listing |
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