AI Article Synopsis
- * Researchers created a novel inhalable treatment using milk exosomes that carry siRNA targeting TGF-β1, which was tested on mice with PF induced by bleomycin (BLM).
- * The treatment improved lung health by reducing inflammation and fibrous tissue buildup, leading to a significant increase in survival rates for the mice, indicating its potential for treating fibrosis-related diseases.
Article Abstract
Pulmonary Fibrosis (PF) is a fatal disease in the interstitial lung associated with high mortality, morbidity, and poor prognosis. Transforming growth factor-β1 (TGF-β1) is a fibroblast-activating protein that promotes fibrous diseases. Herein, an inhalable system was first developed using milk exosomes (M-Exos) encapsulating siRNA against TGF-β1 (MsiTGF-β1), and their therapeutic potential for bleomycin (BLM)-induced PF was investigated. M-siTGF-β1 was introduced into the lungs of mice with PF through nebulization. The collagen penetration effect and lysosomal escape ability were verified in vitro. Inhaled MsiTGF-β1 notably alleviated inflammatory infiltration, attenuated extracellular matrix (ECM) deposition, and increased the survival rate of PF mice by 4.7-fold. M-siTGF-β1 protected lung tissue from BLM toxicity by efficiently delivering specific siRNA to the lungs, leading to TGF-β1 mRNA silencing and epithelial mesenchymal transition pathway inhibition. Therefore, M-siTGF-β1 offers a promising avenue for therapeutic intervention in fibrosis-related disorders.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11267965 | PMC |
| http://dx.doi.org/10.1186/s12951-024-02721-z | DOI Listing |
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