AI Article Synopsis
- Viruses pose significant threats to global health and economies, necessitating a deeper understanding of their infectious states.
- Current methods for studying viruses are largely inadequate, prompting the need for better techniques.
- The new FAIRY assay can quickly distinguish between intact and nonintact enveloped viruses, showcasing its potential for studying viral infectivity and treatment applications.
Article Abstract
Viruses are a considerable threat to global health and place major burdens on economies worldwide. Manufactured viruses are also being widely used as delivery agents to treat (gene therapies) or prevent diseases (vaccines). Therefore, it is vital to study and fully understand the infectious state of viruses. Current techniques used to study viruses are often slow or nonexistent, making the development of new techniques of paramount importance. Here we present a high-throughput and robust, cell-free plate-based assay (FAIRY: Fluorescence Assay for vIRal IntegritY), capable of differentiating intact from nonintact enveloped viruses, i.e, infectious from noninfectious. Using a thiazole orange-terminated polymer, a 99% increase in fluorescence was observed between treated (heat or virucide) and nontreated. The FAIRY assay allowed for the rapid determination of the infectivity of a range of enveloped viruses, highlighting its potential as a valuable tool for the study of viruses and interventions against them.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11323024 | PMC |
| http://dx.doi.org/10.1021/acs.biomac.4c00358 | DOI Listing |
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