Yeast Expression and Activity Assay of RVFV Polymerase.

Methods Mol Biol

State Key Laboratory of Animal Biotech Breeding, College of Biological Sciences, China Agricultural University, Beijing, China.

Published: July 2024

Rift Valley fever (RVF) virus is widespread worldwide and poses a severe threat to human life and property. RVF viral polymerase plays a vital role in the replication and transcription of the virus. Here, we describe how to express and purify this polymerase and perform tests for its in vitro activity assays.

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http://dx.doi.org/10.1007/978-1-0716-3926-9_20DOI Listing

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