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Activation of the BMP2/SMAD4 signaling pathway for enhancing articular cartilage regeneration of mesenchymal stem cells utilizing chitosan/alginate nanoparticles on 3D extracellular matrix scaffold. | LitMetric

AI Article Synopsis

  • This study explored the use of chitosan/alginate nanoparticles with recombinant human bone morphogenetic-2 (rhBMP-2) and a plasmid for enhancing cartilage formation from human bone marrow stem cells.
  • The stem cells were treated with various combinations of biological agents, and the effectiveness was measured using gene expression analysis and staining techniques.
  • Results showed the biological cocktail (BC) significantly boosted cartilage-related gene expression compared to other treatments, indicating its potential for improving cartilage regeneration.

Article Abstract

This study investigated the efficacy of using chitosan/alginate nanoparticles loaded with recombinant human bone morphogenetic-2 (rhBMP-2) and SMAD4 encoding plasmid to enhance the chondrogenesis of human bone marrow mesenchymal stem cells (hBM-MSCs) seeded on an extracellular matrix (ECM). The research treatments included the stem cells treated with the biological cocktail (BC), negative control (NC), hBM-MSCs with chondrogenic medium (MCM), hBM-MSCs with naked rhBMP-2 and chondrogenic medium (NB/C), and hBM-MSCs with naked rhBMP-2 and chondrogenic medium plus SMAD4 encoding plasmid transfected with polyethyleneimine (PEI) (NB/C/S/P). The cartilage differentiation was performed with real-time quantitative PCR analysis and alizarin blue staining. The data indicated that the biological cocktail (BC) exhibited significantly higher expression of cartilage-related genes compared to significant differences with MCM and negative control (NC) on chondrogenesis. In the (NB/C/S/P), the expression levels of SOX9 and COLX were lower than those in the BC group. The expression pattern of the ACAN gene was similar to COL2A1 changes suggesting that it holds promising potential for cartilage regeneration.

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Source
http://dx.doi.org/10.1016/j.ijbiomac.2024.133995DOI Listing

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