The inhibition roles of gene in granulosa cell proliferation and progesterone synthesis of hen ovarian prehierarchical follicles.

1. The proliferation of granulosa cells is vital for the development and recruitment of hen ovarian prehierarchical follicles (PF). The RAB23 protein is a member of the Rab family, belonging to the GTPase family. This study studied the regulatory roles of the gene in PF.2. The expression of RAB23 was significantly increased in granulosa cells (GC) during PF growth and was highest in GC at 6-8 mm diameter ( < 0.05). The RAB23 protein was mainly expressed in the GC, oocytes (OC) as well as somatic cells (SC) of the PF.3. The mRNA expression of , , and was significantly increased in the siRNA group ( < 0.05). Additionally, protein expression of FSHR, CCND1, CYP11A1, HSD3B1 was significantly increased ( < 0.05) after GC were transfected with -specific siRNA. Protein expression of StAR in the siRNA group was numerically higher than that in the positive control (PC) and negative control (NC) groups. The GC proliferation rate and progesterone synthesis of the prehierarchical follicles in hen ovaries were markedly increased ( < 0.05).4.This study revealed that might play an inhibitory role in GC proliferation and progesterone synthesis during the prehierarchical follicles development .