ACS Omega
Institute for Separation and Process Technology, Clausthal University of Technology, Clausthal-Zellerfeld 38678, Germany.
Published: July 2024
Plasmid DNA is an important substance for the pharmaceutical industry. A major challenge in its production is the clarification of the lysate after harvesting. In this work, a novel process for this is demonstrated in an annular centrifugal contactor (ACC) with an aqueous two-phase extraction. The ACC can increase the space-time yield of the clarification step by at least a factor of 2 compared to the horizontal continuous separator. A model for describing and predicting the conditions in the rotor is created for the ACC and calibrated with an accuracy of = 0.94, which is sufficient for prediction in process design and operation. A digital twin (DT), which determines the purity of the phases at the outlet of the ACC, is calibrated with an accuracy of 95% for the light phase and 97% for the heavy phase, which defines in manufacturing operation the safety margin for both phases below the theoretical optimal operation point. By using the DT, an additional increase in productivity of 25% can be achieved in the ACC with model-predictive control.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11256308 | PMC |
http://dx.doi.org/10.1021/acsomega.4c04530 | DOI Listing |
Shock
January 2025
Department of Industrial and Systems Engineering, University of Florida, P.O. Box 116595, Gainesville, FL, 32611, USA.
Understanding clinical trajectories of sepsis patients is crucial for prognostication, resource planning, and to inform digital twin models of critical illness. This study aims to identify common clinical trajectories based on dynamic assessment of cardiorespiratory support using a validated electronic health record data that covers retrospective cohort of 19,177 patients with sepsis admitted to ICUs of Mayo Clinic Hospitals over eight-year period. Patient trajectories were modeled from ICU admission up to 14 days using an unsupervised machine learning two-stage clustering method based on cardiorespiratory support in ICU and hospital discharge status.
View Article and Find Full Text PDFInt J Biol Macromol
January 2025
School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, China. Electronic address:
Myricetin has a significant role in pharmacology, specifically in traditional Chinese medicine. The most intriguing pharmacological action of myricetin consists of its multi-pathway anticancer effects. Therefore, rapid and selective isolation of myricetin from garlic and apple juices has notable pharmacological benefits.
View Article and Find Full Text PDFSoft Robot
January 2025
Department of Mechanical and Nuclear Engineering, Khalifa University, Abu Dhabi, UAE.
The inherent challenges of robotic underwater exploration, such as hydrodynamic effects, the complexity of dynamic coupling, and the necessity for sensitive interaction with marine life, call for the adoption of soft robotic approaches in marine exploration. To address this, we present a novel prototype, ZodiAq, a soft underwater drone inspired by prokaryotic bacterial flagella. ZodiAq's unique dodecahedral structure, equipped with 12 flagella-like arms, ensures design redundancy and compliance, ideal for navigating complex underwater terrains.
View Article and Find Full Text PDFCureus
December 2024
Department of Ophthalmology, Broward Health, Fort Lauderdale, USA.
This literature review explores the emerging role of digital twin (DT) technology in ophthalmology, emphasizing its potential to revolutionize personalized medicine. DTs integrate diverse data sources, including genetic, environmental, and real-time patient data, to create dynamic, predictive models that enhance risk assessment, surgical planning, and postoperative care. The review highlights vital case studies demonstrating the application of DTs in improving the early detection and management of diseases such as glaucoma and age-related macular degeneration.
View Article and Find Full Text PDFBMC Med
January 2025
Liverpool Centre for Cardiovascular Science at University of Liverpool, Liverpool John Moores University and Liverpool Heart & Chest Hospital, Liverpool, UK.
Background: Polypharmacy (i.e., treatment with ≥ 5 drugs) is common in patients with atrial fibrillation (AF) and has been associated with suboptimal management and worse outcomes.
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