AI Article Synopsis
- Single-cell spatial analysis of proteins is crucial for gaining insights into biology, especially in cancer research.
- The text outlines an automated protocol for multi-slide immunofluorescence staining and imaging of human head and neck cancer samples using PhenoCycler-Fusion 2.0 technology.
- It includes detailed steps for tissue preparation, staining with immunophenotyping markers, and subsequent analysis procedures, with more specifics available in a referenced study by Jhaveri et al.
Article Abstract
Single-cell spatial analysis of proteins is rapidly becoming increasingly important in revealing biological insights. Here, we present a protocol for automated high-plex multi-slide immunofluorescence staining and imaging of human head and neck cancer formalin-fixed paraffin-embedded (FFPE) sections using PhenoCycler-Fusion 2.0 technology. We describe steps for preparing human head and neck cancer FFPE tissues, staining with a panel of immunophenotyping markers, and Flow Cell assembly. We then detail procedures for setting up for a PhenoCycler-Fusion run, post-run Flow Cell removal, and downstream analyses. For complete details on the use and execution of this protocol, please refer to Jhaveri et al..
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Source |
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11314888 | PMC |
| http://dx.doi.org/10.1016/j.xpro.2024.103226 | DOI Listing |
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