AI Article Synopsis
- An enzyme-free fluorescent aptasensor was developed for the sensitive detection of lead (Pb) ions, a dangerous pollutant in the environment and food.
- The aptasensor uses a nanocomposite made of zeolitic imidazolate frameworks-8 and gold nanoparticles to quench the fluorescence of a carboxyfluorescein signal reporter, with a selective binding mechanism that enhances detection.
- This method accurately measures Pb concentrations from 1 pM to 1 nM, with a very low detection limit of 0.24 pM, and can be used on various samples such as fish, shrimp, tap water, milk, and serum, making it a promising tool for food safety checks.
Article Abstract
Herein, an enzyme-free fluorescent aptasensor was introduced for the ultrasensitive quantification of lead (Pb) ion as a hazardous pollutant of the environment and foodstuffs. A nanocomposite of zeolitic imidazolate frameworks-8 and gold nanoparticles (ZIF-8@AuNPs) was utilized as an efficient quencher of the fluorescence intensity of carboxyfluorescein (FAM) signal reporter. The establishment of a hybrid structure between attached aptamer on ZIF-8@AuNPs nanocomposite, and its FAM-tagged complementary (CP) strand decreased the fluorescence response. The preferential binding between the aptamer and Pb released CP strands, which retrieved the fluorescence signal. The aptasensor could assess Pb in the linear concentration range of 1 pM-1 nM with a detection limit (LOD) of 0.24 pM. Besides, it could quantify Pb in various samples, including fish, shrimp, tap water, milk, and serum samples. The developed aptasensor with the superiorities of easiness, cost-effectiveness, easy-to-operate, and rapidness is promising for controlling marine foodstuff safety.
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| http://dx.doi.org/10.1016/j.aca.2024.342893 | DOI Listing |
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