sp. nov., isolated from intestine of mealworm.

A bacterial strain designated PU5-4 was isolated from the mealworm (the larvae of ) intestines. It was identified to be Gram-stain-negative, strictly aerobic, rod-shaped, non-motile, and non-spore-forming. Strain PU5-4 was observed to grow at 10-40 °C, at pH 7.0-10.0, and in the presence of 0-3.0 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain PU5-4 should be assigned to the genus . The 16S rRNA gene sequence similarity analysis showed that strain PU5-4 was closely related to the type strains of DSM 22361 (98.49 %), NYYP31 (98.11 %), NCCP 698 (97.69 %) and HAL-9 (95.73 %). The predominant isoprenoid quinone is MK-7. The major fatty acids were identified as iso-C, iso-C3-OH and summed feature 3 (C 7 and/or C 6) and summed feature 9 (iso-C 9). The polar lipids are phosphatidylethanolamine, one unidentified phospholipid, and six unidentified lipids. The genomic DNA G+C content of strain PU5-4 is 40.24 mol%. The average nucleotide identity of strain PU5-4 exhibited respective values of 73.88, 73.37, 73.36 and 70.84 % comparing to the type strains of DSM 22361, NCCP 698, NYYP31 and HAL-9, which are below the cut-off level (95-96 %) for species delineation. Based on the above results, strain PU5-4 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is PU5-4 (=CGMCC 1.61908=JCM 36663).