AI Article Synopsis

  • Asthma is a complex disease causing chronic inflammation in airways, and Huashanshen dripping pills (HSS) are used to relieve asthma symptoms like cough and phlegm but the exact mechanism is unclear.* -
  • The study utilized network pharmacology and molecular techniques to predict and validate HSS's therapeutic effects on asthma in a mouse model, revealing its impact on reducing inflammation and improving lung function.* -
  • Findings show that HSS lowers levels of inflammatory cytokines and inhibits key proteins involved in inflammation, specifically targeting the NF-κB signaling pathway, suggesting potential for asthma treatment.*

Article Abstract

Objectives: Asthma is a heterogeneous disease characterized by chronic airway inflammation. Huashanshen dripping pills (HSS) are commonly utilized for relieving asthma, relieving cough, and expelling phlegm. At present, the molecular mechanism against airway inflammation remains unclear.

Methods: In this study, network pharmacology, molecular docking technology, and molecular dynamic simulation were used to predict the therapeutic pathways of HSS for asthma. The ovalbumin-induced mouse model was used to further validate the prediction by RT-qPCR, western blot, immunofluorescence, and related methods.

Key Findings: The findings indicate that HSS improves lung function and relieves lung inflammation by reducing inflammatory cell infiltration around the bronchus and reducing eosinophilic counts in bronchoalveolar lavage fluid (BALF). In addition, it lowers the levels of inflammatory cytokines and the expression levels of interleukin-4, interleukin-5, and interleukin-13 mRNA. HSS also inhibits the phosphorylation and nuclear translocation of NF-κB p65 protein.

Conclusions: All results suggested that HSS can decrease airway inflammation in asthmatic mice by inhibiting NF-κB signaling pathway. This finding will shed light on how it can be used to treat asthma.

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Source
http://dx.doi.org/10.1093/jpp/rgae081DOI Listing

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