AI Article Synopsis
- - A novel fungus species was identified from mangrove soil, leading to the discovery of four new -lactam alkaloids (talaroilactams A-D) along with two known compounds (harzianic and isoharzianic acid) through morphological observation and advanced analysis techniques.
- - The structures of these compounds were confirmed using calculations and spectroscopic methods, and a biosynthetic pathway was proposed for one of the compounds, which showed strong cytotoxic effects against HepG2 liver cancer cells.
- - Network pharmacology analysis revealed that the antihepatocellular carcinoma activity of this compound is linked to processes such as proteolysis and inflammation regulation, suggesting potential for new treatments derived from mangrove fungi
Article Abstract
The fungus , isolated from the mangrove soil, was characterized as a novel species by morphology observation and phylogenetic analyses. Four new -lactam alkaloids talaroilactams A-D (-) and two reported compounds harzianic acid () and isoharzianic acid () were identified from the fungus WHUF0341, assisted by OSMAC along with molecular networking approaches. Their structures were determined through ECD calculations and spectroscopic analyses. Moreover, the biosynthetic route of - was also proposed. Compound displayed potent cytotoxicity against HepG2 cell lines, with an IC value of 10.75 ± 1.11 μM. In addition, network pharmacology was employed to dissect the probable mechanisms contributing to the antihepatocellular carcinoma effects of compound , revealing that cytotoxicity was mainly associated with proteolysis, negative regulation of autophagy, inflammatory response, and the renin-angiotensin system. These results not only expanded the chemical space of natural products from the mangrove associated fungi but also afforded promising lead compounds for developing the antihepatocellular carcinoma agents.
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