AI Article Synopsis
- Feline coronavirus (FCoV) is a major cause of death in cats, prompting the development of a vaccine using FCoV virus-like particles (VLPs) with specific proteins and adjuvants.
- Results showed that mice and FCoV-negative cats produced high levels of specific antibodies after vaccination, indicating a strong immune response.
- In FCoV-positive cats, booster shots led to reduced viral load in feces and negative conversion of the virus in some cases, suggesting the vaccine's potential effectiveness in preventing FCoV infection.
Article Abstract
Feline coronavirus (FCoV) infection is a leading cause of death in cats. In this study, we produced FCoV-I virus-like particles (VLPs) containing E, M, N, and S proteins using a baculovirus expression system and mixed VLPs with the adjuvants MF59 and CpG 55.2 to prepare an VLP/MF59/CpG vaccine. After immunization of mice with the vaccine, IgG specific antibodies titers against S and N proteins increased to 1:12,800, and IFN-γ and IL-4 splenocytes were significantly increased. Following immunization of FCoV-negative cats, the S protein antibodies in immunized cats (5/5) increased significantly, with a peak of 1:12,800. Notably, after booster vaccination in FCoV-positive cats, a significant reduction in viral load was observed in the feces of partial cats (4/5), and the FCoV-I negative conversion was found in two immunized cats (2/5). Therefore, the VLP/MF59/CpG vaccine is a promising candidate vaccine to prevent the FCoV infection.
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| http://dx.doi.org/10.1016/j.micpath.2024.106795 | DOI Listing |
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