AI Article Synopsis
- Memory B cells are crucial for creating long-term immunity and improving vaccine effectiveness against specific pathogens.
- Disruptions in the formation of memory B cells during ongoing infections need to be understood for the development of new treatments.
- The text describes a protocol for isolating and analyzing LCMV-specific memory B cells during both acute and chronic infections, utilizing techniques like antigen tetramer enrichment and flow cytometry.
Article Abstract
Memory B cells are central to the establishment of immunological memory, providing long-term protection against specific pathogens and playing a vital role in the efficacy of vaccines. Understanding how memory B cell formation is disrupted during persistent infection is essential for new therapeutics. Lymphocytic choriomeningitis virus (LCMV) is an ideal model for investigating memory B cells in acute versus chronic infection. This protocol details techniques to isolate, enrich, and examine LCMV-specific memory B cells in both acute and chronic LCMV infection. Using an antigen tetramer enrichment system and flow cytometry, this method assesses low-frequency, polyclonal antigen-specific memory B cells.
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| http://dx.doi.org/10.1007/978-1-0716-3950-4_9 | DOI Listing |
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