sp. nov., isolated from mountain soil.

Int J Syst Evol Microbiol

Department of Life Science, College of Natural Sciences, Kyonggi University, Suwon, Kyonggi-Do 16227, Republic of Korea.

Published: July 2024

Gram-stain-positive, aerobic, rod-shaped strains, YJM1 and YJM12S, were isolated from Maebong Mountain, Dogok-dong, Gangnam-gu, Seoul, Republic of Korea. Strains YJM1 and YJM12S exhibited growth at 5-35 °C (optimum, 20-30 °C) and pH 6-9 (optimum, pH 7) and in 0-4 % (w/v) NaCl. Strains YJM1 and YJM12S showed highest 16S rRNA gene sequence similarity to the following members of the genus : A33 (98.3 %/98.2 % similarity), NBRC 107840 (98.2 %/98.1 %), KV-653 (97.3 %), KV-651 (97.3 %), and NBRC 12137 (97.2 %). The strains grew well on Reasoner's 2A, nutrient, Mueller-Hinton, yeast-dextrose, and glucose-peptone-meat extract agars. The major polar lipids of strain YJM1 were phosphatidylglycerol, diphosphatidylglycerol, and phosphatidylinositol. The primary respiratory quinone of strain YJM1 was MK-9(H), and the major fatty acids of strains YJM1 and YJM12S were anteiso-C, anteiso-C, iso-C, and iso-C. The DNA G+C content, based on the whole genome sequence of strain YJM1, was 68.3 mol%. Average nucleotide identity values and digital DNA-DNA hybridization values between strain YJM1 and the reference strains ranged from 75.0 to 92.7 % and from 21.0 to 65.3 %, respectively. Strain YJM1 exhibited antimicrobial activity against and . Considering the chemotaxonomic, phenotypic, genotypic, and phylogenetic results, we propose the strain YJM1 represents a novel species in the genus and suggest the name sp. nov. (type strain YJM1=KACC 23300=JCM 36483).

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http://dx.doi.org/10.1099/ijsem.0.006459DOI Listing

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