AI Article Synopsis
- Researchers introduced a new method called COMPASS that improves the detection of cancer biomarkers in serum by using magnetic nanoparticles and a panel of aptamers for better accuracy.
- The study focused on non-small cell lung cancer (NSCLC) and identified a panel of four biomarkers (HE4, NSE, AFP, and VEGF165) as the most effective for diagnosis.
- With high diagnostic accuracy rates of 95.56% and 89.58% in different testing groups, COMPASS offers a promising approach for efficient and scalable cancer screening.
Article Abstract
Detection of serum protein biomarkers is extremely challenging owing to the superior complexity of serum. Here, we report a method of proteome fishing from the serum. It uses a magnetic nanoparticle-protein corona and a multiplexed aptamer panel, which we incubated with the nanoparticle-protein corona for biomarker recognition. To transfer protein biomarker detection to aptamer detection, we established a CRISPR/Cas12a-based orthogonal multiplex aptamer sensing (COMPASS) platform by profiling the aptamers of protein corona with clinical nonsmall cell lung cancer (NSCLC) serum samples. Furthermore, we determined the four out of nine (FOON) panel (including HE4, NSE, AFP, and VEGF165) to be the most cost-effective and accurate panel for COMPASS in NSCLC diagnosis. The diagnostic accuracy of NSCLC by the FOON panel with internal and external cohorts was 95.56% (ROC-AUC = 99.40%) and 89.58% (ROC-AUC = 95.41%), respectively. Our developed COMPASS technology circumvents the otherwise challenging multiplexed serum protein amplification problem and avoids aptamer degradation in serum. Therefore, this novel COMPASS could lead to the development of a facile, cost-effective, intelligent, and high-throughput diagnostic platform for large-cohort cancer screening.
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| http://dx.doi.org/10.1021/jacs.4c03061 | DOI Listing |
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