Background And Objectives: is known as the main cause of gastrointestinal diseases including gastritis, gastric ulcer and stomach cancer. Serodiagnosis of infection is a noninvasive and rapid method but the efficiency of this method is highly dependent to the antigens used. This study evaluated the efficacy of recombinant UreB-Omp18 and FliD for serodiagnosis of infection.
Materials And Methods: The genes encoding for and was amplified by PCR and cloned into pET-22b and pET-28a vectors. The constructs were expressed in BL21 and purified by affinity chromatography. The antigenic properties and diagnostic potential of the recombinant proteins were analysed by immunoblotting and ELISA, respectively.
Results: The recombinant UreB-Omp18 and FliD with molecular weights of 48 kDa and 25 kDa were observed on SDS-PAGE and purified by the Ni-NTA column. The ELISA results showed that the sensitivity and specificity of recombinant UreB-Omp18 protein in serodiagnosis of infection were 89% and 83%, respectively. Also, the sensitivity and specificity of the recombinant FliD protein were calculated to be 91% and 76%, respectively.
Conclusion: The results indicated that the recombinant UreB-Omp18 and FliD could diagnose infection with high sensitivity and specificity.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11245343 | PMC |
http://dx.doi.org/10.18502/ijm.v16i3.15768 | DOI Listing |
Iran J Microbiol
June 2024
Department of Microbiology, Faculty of Basic Sciences, Jahrom Branch, Islamic Azad University, Jahrom, Iran.
Background And Objectives: is known as the main cause of gastrointestinal diseases including gastritis, gastric ulcer and stomach cancer. Serodiagnosis of infection is a noninvasive and rapid method but the efficiency of this method is highly dependent to the antigens used. This study evaluated the efficacy of recombinant UreB-Omp18 and FliD for serodiagnosis of infection.
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