AI Article Synopsis
- 5-MeO-AMT is a new psychoactive substance that is gaining attention due to its hallucinogenic and euphoric effects, prompting research into its metabolism.
- In a study involving rats, researchers injected 5-MeO-AMT and analyzed urine samples using advanced mass spectrometry to identify its metabolites.
- Three key metabolites were discovered, with the main metabolic processes identified as O-demethylation, hydroxylation, and acetylation, providing valuable insights for detecting potential toxicity related to 5-MeO-AMT.
Article Abstract
Objective: 5-Methoxy-α-Methyltryptamine (5-MeO-AMT) is a new psychoactive substance which is abused due to its hallucinogenic and euphoric effects. This study aimed to study the metabolic characteristics of 5-MeO-AMT.
Methods: Five rats were given intraperitoneal injection at a dose of 50 mg/kg of 5-MeO-AMT, and their urine was subsequently collected at different times within 7 days. Ultra-high performance liquid chromatographytandem high-resolution mass spectrometry (UPLC-LTQ-Orbitrap) was used to detect the precise molecular weight and fragment ions of 5-MeO-AMT and its possible metabolites in the urine sample extracted with benzene-ethyl acetate.
Results: Three metabolites, including OH-5-MeO-AMT, α-Me-5-HT, and N-Acetyl-5-MeO-AMT were identified in rats' urine. The major metabolic pathways involved O-demethylation, hydroxylation of indole ring, and Acetylation on aliphatic amines.
Conclusion: The results of this study are an important reference for the identification and screening of toxicants of 5-MeO-AMT.
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| http://dx.doi.org/10.2174/0113892002295551240628061732 | DOI Listing |
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