AI Article Synopsis
- Listeria monocytogenes shows genetic similarities with non-pathogenic Listeria species and is known for surviving extreme conditions like acidity, cold, and high salt.
- This study analyzed 65 strains of Listeria from various environments to evaluate their resistance to stress and identified two categories: stress-resistant (56 strains) and stress-sensitive (9 strains).
- The study also examined specific virulence and stress-related genes, finding that certain genes related to low temperatures and salt resistance were more common in the stress-resistant strains.
Article Abstract
Genetically, Listeria monocytogenes is closely related to non-L. monocytogenes (L. innocua, L. welshimeri, L. grayi, L. aquatica, and L. fleischimannii). This bacterium is well known for its resistance to harsh conditions including acidity, low temperatures, and high salt concentrations. This study explored the responses of 65 Listeria strains to stress conditions and characterized the prevalence of stress-related genes. The 65 Listeria strains were isolated from different environments and their viability was assessed in four different tests: independent tests for pH 3, 1 °C, and 5 % salt concentration and multiple resistance tests that combined pH 3, 1 °C, 5 % salt. From the data, the 65 strains were categorized into stress-resistant (56) or stress-sensitive groups (9), with approximately 4 log CFU/mL differences. The PCR assay analyzed the prevalence of two virulence genes prfA and inlA, and eight stress-related genes: three acid (gadB, gadC, and atpD), two low temperature (betL and opuCA) and three salt resistance genes (flaA, cysS, and fbp). Two low temperature (bet and opuCA) and salt resistance (fbp) genes were more prevalent in the stress-resistant strains than in the stress-sensitive Listeria group.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.micpath.2024.106793 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Novel primers drive accurate SYBR Green PCR detection of Listeria monocytogenes and Listeria innocua in cultures and mushrooms.
Sci Rep
January 2025
Microbial Safety Division, National Institute of Agricultural Sciences, Rural Development Administration, Wanju, 55365, Republic of Korea.
The close genetic resemblance between Listeria monocytogenes and Listeria innocua, combined with their presence in similar environments, poses challenges for species-specific detection in food products. Ensuring food safety through microbiological standards necessitates reliable detection of pathogens like L. monocytogenes and L.
View Article and Find Full Text PDFMultilocus Sequence Typing and Antimicrobial Susceptibility of Isolated from Foods Surveyed in Kosovo.
Microorganisms
November 2024
Department of Biochemistry, Genetics and Microbiology, University of Pretoria, Private Bag X20, Hatfield, Pretoria 0028, South Africa.
In the absence of data on the reporting of resistance to antibiotics, we sought to determine which clonal complexes (CCs)/sequence types (STs) circulate in the food chain in Kosovo and to determine their antibiogram profiles to a panel of 18 antibiotics. From a total of 114 isolates, 21 different typical STs were identified by multilocus sequence typing (MLST). Each isolate derived from the food categories was subjected to tests to verify its susceptibility to the selected antibiotics according to the designed Sensititre GPN3F panel.
View Article and Find Full Text PDFScreening and Genomic Profiling of Antimicrobial Bacteria Sourced from Poultry Slaughterhouse Effluents: Bacteriocin Production and Safety Evaluation.
Genes (Basel)
December 2024
Departamento de Nutrición y Ciencia de los Alimentos (NUTRYCIAL), Sección Departamental de Nutrición y Ciencia de los Alimentos (SD-NUTRYCIAL), Facultad de Veterinaria, Universidad Complutense de Madrid (UCM), Avenida Puerta de Hierro, s/n, 28040 Madrid, Spain.
Antimicrobial-resistant (AMR) pathogens represent a serious threat to public health, particularly in food production systems where antibiotic use remains widespread. As a result, alternative antimicrobial treatments to antibiotics are essential for effectively managing bacterial infections. This study aimed to identify and characterize novel antimicrobial peptides produced by bacteria, known as bacteriocins, as well as to recognize safe bacteriocin-producing strains, sourced from poultry slaughterhouse effluents.
View Article and Find Full Text PDFNatural Mutation of PrfA K10N/T151A Enhances Serotype 4h Virulence.
Foodborne Pathog Dis
January 2025
College of Biological Sciences and Technology, Yangzhou University, Yangzhou, China.
PrfA is a key virulence regulator for (Lm) responding to host environment. Here we report that the natural mutation in PrfA enhanced the pathogenicity of hypervirulent serotype 4h . We characterized the phylogenetic tree of PrfA, and found that PrfA prevalently distributed in all serotype 4h isolates.
View Article and Find Full Text PDFModelling growth of two monocytogenes strains, persistent and non-persistent: Effect of temperature.
Heliyon
December 2024
Department of Microbiology, Molecular Biology and Biotechnology, Food Research Institute, National Agricultural and Food Centre, Priemyselná 4, 824 75, Bratislava, Slovakia.
Better growth is a phenotypic trait that can contribute to persistence of in food processing environments. To test the hypothesis objectively, persistent and non-persistent strains were selected and grown in different media to gain reliable quantitative growth characteristics. In this study, the effect of temperature in the range from 6 °C to 43 °C on the planktonic growth of genotypically and phenotypically different strains LM9611-19 (LM-P, persistent) and LM120/5 (LM-S, sporadic - potentially non-persistent) in Tryptone Soy Broth (TSB) and in semi-synthetic cheese medium (SCM) was investigated.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!