A high-quality chromosome-level genome assembly of the endangered tree Kmeria septentrionalis.

Sci Data

Guangxi Key Laboratory of Sericulture Ecology and Applied Intelligent Technology, School of Chemistry and Bioengineering, Hechi University, Hechi, 546300, China.

Published: July 2024

AI Article Synopsis

  • Kmeria septentrionalis is a critically endangered tree species native to Guangxi, China, and is on the IUCN's Red List, highlighting its conservation urgency.
  • The study offers a detailed chromosome-level genome assembly of the tree, revealing a genome size of 2.54 Gb and a high completeness score, conferring valuable genetic insights.
  • This genomic resource not only supports conservation efforts but also facilitates evolutionary research within the Magnoliaceae family.

Article Abstract

Kmeria septentrionalis is a critically endangered tree endemic to Guangxi, China, and is listed on the International Union for Conservation of Nature's Red List. The lack of genetic information and high-quality genome data has hindered conservation efforts and studies on this species. In this study, we present a chromosome-level genome assembly of K. septentrionalis. The genome was initially assembled to be 2.57 Gb, with a contig N50 of 11.93 Mb. Hi-C guided genome assembly allowed us to anchor 98.83% of the total length of the initial contigs onto 19 pseudochromosomes, resulting in a scaffold N50 of 135.08 Mb. The final chromosome-level genome, spaning 2.54 Gb, achieved a BUSCO completeness of 98.9% and contained 1.67 Gb repetitive elements and 35,927 coding genes. This high-quality genome assembly provides a valuable resource for understanding the genetic basis of conservation-related traits and biological properties of this endangered tree species. Furthermore, it lays a critical foundation for evolutionary studies within the Magnoliaceae family.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11246460PMC
http://dx.doi.org/10.1038/s41597-024-03617-1DOI Listing

Publication Analysis

Top Keywords

genome assembly
16
chromosome-level genome
12
endangered tree
12
kmeria septentrionalis
8
high-quality genome
8
genome
7
high-quality chromosome-level
4
assembly
4
assembly endangered
4
tree kmeria
4

Similar Publications

Genome-wide maps of highly-similar intrachromosomal repeats that can mediate ectopic recombination in three human genome assemblies.

HGG Adv

December 2024

International Laboratory for Human Genome Research, Laboratorio Internacional de Investigación sobre el Genoma Humano, Universidad Nacional Autónoma de México, Juriquilla, Querétaro, México. Electronic address:

Repeated sequences spread throughout the genome play important roles in shaping the structure of chromosomes and facilitating the generation of new genomic variation through structural rearrangements. Several mechanisms of structural variation formation use shared nucleotide similarity between repeated sequences as substrate for ectopic recombination. We performed genome-wide analyses of direct and inverted intrachromosomal repeated sequence pairs with >200bp and >80% sequence identity in three human genome assemblies, GRCh37, GRCh38, and the T2T-CHM13 alternate assembly.

View Article and Find Full Text PDF

Objectives: This study aims to generate a de novo complete whole-genome assembly of Pseudomonas sp. strain HOU2, which is an endophytic bacterium isolated from dangshen roots that shows to improve the growth of in vitro dangshen plants. Further investigation of the whole genome of Pseudomonas sp.

View Article and Find Full Text PDF

Background: Variants in have been reported to be associated with Leigh syndrome. However, further expansion of the -phenotype and variants spectrum of -related Leigh syndrome are still required.

Methods: Two patients diagnosed with Leigh syndrome were recruited, and whole-exome sequencing was performed to identify the genetic variants responsible for the abnormal gait, dystonia, and bilateral basal ganglia lesions, followed by validation using Sanger sequencing.

View Article and Find Full Text PDF

Meta-assembly of genomic associations to identify cattle fat depot candidate genes and pleiotropic effects.

BMC Genomics

December 2024

School of Animal and Veterinary Sciences, The University of Adelaide, Roseworthy Campus, Roseworthy, South Australia, 5371, Australia.

Background: Fat traits in cattle are considered important due to their contribution to beef eating quality and carcass economic value. Discovering the genes controlling fat traits in cattle will enable better selection of these traits, but identifying these genes in individual experiments has proven difficult. Compared to individual experiments, meta-analyses allow greater statistical power for detecting quantitative trait loci and identifying genes that influence single and multiple economically important fat traits.

View Article and Find Full Text PDF

Effects of ALS-associated 5'tiRNA on the transcriptomic and proteomic profile of primary neurons in vitro.

Exp Neurol

December 2024

Department of Physiology and Medical Physics, Royal College of Surgeons in Ireland, 123 St. Stephen's Green, Dublin, Ireland; FutureNeuro Research Ireland Centre, Royal College of Surgeons in Ireland, Dublin, Ireland. Electronic address:

tRNA-derived stress-induced RNAs (tiRNAs) are a new class of small non-coding RNA that have emerged as important regulators of cellular stress responses. tiRNAs are derived from specific tRNA cleavage by the stress-induced ribonuclease angiogenin (ANG). Loss-of-function mutations in the ANG gene are linked to amyotrophic lateral sclerosis (ALS), and elevated levels of specific tiRNAs were recently identified in ALS patient serum samples.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!