Prokaryotic Expression and Functional Verification of Antimicrobial Peptide LR.

Int J Mol Sci

Engineering Research Center, The Chinese Ministry of Education for Bioreactor and Pharmaceutical Development, College of Life Sciences, Jilin Agricultural University, Changchun 130118, China.

Published: June 2024

The antimicrobial peptide LR (LLRLLRRGGRRLLRLL-NH2) was designed and chemically synthesized in a study conducted by Jia et al. Gram-negative bacteria were found to be sensitive to LR and exhibited a high therapeutic index. Genetic engineering methods were used to create the prokaryotic fusion expression vector pQE-GFP-LR, and the resulting corresponding fusion protein GFP-LR was subsequently expressed and purified. The precursor GFP was then removed by TEV proteolysis, and pure LR was obtained after another round of purification and endotoxin removal. The prokaryotic-expressed antimicrobial peptide LR displays a broad-spectrum antibacterial effect on Gram-negative bacteria, and its minimum inhibitory activity (MIC) against Escherichia coli can reach 2 μg/mL. Compared to the chemically synthesized LR the prokaryotic-expressed LR exhibits similar temperature, pH, salt ion, serum stability, and cell selectivity. Furthermore, prokaryotic-expressed LR showed excellent therapeutic effects in both the infection model of cell selectivity and no embryotoxicity in a infection model. The mechanism by which LR causes bacterial death was found to be the disruption of the Gram-negative cell membrane.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11241267PMC
http://dx.doi.org/10.3390/ijms25137072DOI Listing

Publication Analysis

Top Keywords

antimicrobial peptide
12
chemically synthesized
8
gram-negative bacteria
8
cell selectivity
8
infection model
8
prokaryotic expression
4
expression functional
4
functional verification
4
verification antimicrobial
4
peptide antimicrobial
4

Similar Publications

Unfolding the Potential of Pyrrole- and Indole-Based Allylidene Hydrazine Carboximidamides as Antimicrobial Agents.

ACS Infect Dis

January 2025

Pharmaceutical Chemistry Research Laboratory, Department of Pharmacy, Birla Institute of Technology and Science Pilani, Pilani Campus, Pilani, Vidya Vihar 333031, (RJ) India.

Antimicrobial drug resistance is a significant global health challenge, causing hundreds of thousands of deaths annually and severely impacting healthcare systems worldwide. Several reported antimicrobial compounds have a guanidine motif, as the positive charge on guanidine promotes cell lysis. Therefore, pyrrole- and indole-based allylidene hydrazine carboximidamide derivatives with guanidine motifs are proposed as antimicrobial agents that mimic cationic antimicrobial peptides (CAMPs).

View Article and Find Full Text PDF

Genome sequence data of GM2: a lipopeptide producer.

Microbiol Resour Announc

January 2025

Department of Microbiology, Institute of Fundamental Medicine and Biology, Kazan (Volga Region) Federal University, Kazan, Russia.

We present the findings from the genome-sequencing project of GM2, sourced from rhizospheric soil and renowned for its lipopeptide production. The genome spans 4,216,713 base pairs with an average G + C content of 43,6%.

View Article and Find Full Text PDF

The emergence of multidrug-resistant (MDR) pathogens, coupled with the limited effectiveness of existing antibiotics in eradicating biofilms, presents a significant threat to global health care. This critical situation underscores the urgent need for the discovery and development of antimicrobial agents. Recently, peptide-derived antimicrobial nanomaterials have shown promise in combating such infections.

View Article and Find Full Text PDF

Cryo-EM structure of the conjugation H-pilus reveals the cyclic nature of the TrhA pilin.

bioRxiv

December 2024

Rutherford Appleton Laboratory, Research Complex at Harwell, Didcot, Oxfordshire, UK.

Conjugation, the major driver of the spread of antimicrobial resistance genes, relies on a conjugation pilus for DNA transfer. Conjugative pili, such as the F-pilus, are dynamic tubular structures, composed of a polymerized pilin, that mediate the initial donor-recipient interactions, a process known as mating pair formation (MPF). IncH are low-copy-number plasmids, traditionally considered broad host range, which are found in bacteria infecting both humans and animals.

View Article and Find Full Text PDF

Bacterial infections pose a threat to human and animal health, and the formation of biofilm exacerbates the microbial threat. New antimicrobial agents to address this challenge are much needed. In this study, several new amphoteric compounds derived from the natural product coumarin were designed and synthesized by mimicking the structure and function of antimicrobial peptides.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!