Kunitz trypsin inhibitor genes play important roles in stress resistance. In this study, we investigated cloned from and its effect on tobacco. was introduced into the tobacco cultivar NC89 using -mediated transformation. Six -overexpressing lines were obtained. Transgenic and wild-type tobacco plants were then compared for photosynthetic characteristics and endogenous hormone levels. Transgenic tobacco showed minor changes in chlorophyll content, fluorescence, and photosynthetic functions. However, the maximum photochemical efficiency (/) increased significantly while intercellular CO concentration () decreased significantly. Stomatal size and hormone content (indole-3-acetic acid, zeatin riboside, gibberellin, and indole-3-propionic acid) were reduced, while brassinosteroid content increased. Random forest regression revealed that overexpression had the biggest impact on carotenoid content, initial fluorescence, , stomatal area, and indole-3-acetic acid. Overall, overexpression minimally affected chlorophyll synthesis and photosynthetic system characteristics but influenced stomatal development and likely enhanced the antioxidant capacity of tobacco. These findings provide a basis for future in-depth research on .
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| http://dx.doi.org/10.3390/plants13131867 | DOI Listing |
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